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Cellulose-binding activity of a 21-kDa endo-ß-1,4-glucanase lacking cellulose-binding domain and its synergy with other cellulases in the digestive fluid of Aplysia kurodai
https://tokushima-u.repo.nii.ac.jp/records/2006064
https://tokushima-u.repo.nii.ac.jp/records/2006064d7990fb1-13c1-4664-b85b-b376562358ac
| 名前 / ファイル | ライセンス | アクション |
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pone_13_11_e0205915.pdf (5.11 MB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||
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| 公開日 | 2019-01-11 | |||||||||||||||||
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| アクセス権 | open access | |||||||||||||||||
| 資源タイプ | ||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||
| 出版社版DOI | ||||||||||||||||||
| 関連識別子 | https://doi.org/10.1371/journal.pone.0205915 | |||||||||||||||||
| 関連名称 | 10.1371/journal.pone.0205915 | |||||||||||||||||
| 出版タイプ | ||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||
| タイトル | ||||||||||||||||||
| タイトル | Cellulose-binding activity of a 21-kDa endo-ß-1,4-glucanase lacking cellulose-binding domain and its synergy with other cellulases in the digestive fluid of Aplysia kurodai | |||||||||||||||||
| タイトル別表記 | ||||||||||||||||||
| その他のタイトル | Proccessive ß-1,4-endoglucanase of sea hare | |||||||||||||||||
| 著者 |
辻, 明彦
× 辻, 明彦
WEKO
121
× 湯浅, 恵造× 淺田, 元子
WEKO
645
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| 抄録 | ||||||||||||||||||
| 内容記述 | Endo-s-1,4-glucanase AkEG21 belonging to glycosyl hydrolase family 45 (GHF45) is the most abundant cellulase in the digestive fluid of sea hare (Aplysia kurodai). The specific activity of this 21-kDa enzyme is considerably lower than those of other endo s-1,4-glucanases in the digestive fluid of A. kurodai, therefore its role in whole cellulose hydrolysis by sea hare is still uncertain. Although AkEG21 has a catalytic domain without a cellulose binding domain, it demonstrated stable binding to cellulose fibers, similar to that of fungal cellobiohydrolase (CBH) 1 and CBH 2, which is strongly inhibited by cellohexaose, suggesting the involvement of the catalytic site in cellulose binding. Cellulose-bound AkEG21 hydrolyzed cellulose to cellobiose, cellotriose and cellotetraose, but could not digest an external substrate, azo-carboxymethyl cellulose. Cellulose hydrolysis was considerably stimulated by the synergistic action of cellulose-bound AkEG21 and AkEG45, another s-1,4-endoglucanase present in the digestive fluid of sea hare; however no synergy in carboxymethylcellulose hydrolysis was observed. When AkEG21 was removed from the digestive fluid by immunoprecipitation, the cellulose hydrolyzing activity of the fluid was significantly reduced, indicating a critical role of AkEG21 in cellulose hydrolysis by A. kurodai. These findings suggest that AkEG21 is a processive endoglucanase functionally equivalent to the CBH, which provides a CBH-independent mechanism for the mollusk to digest seaweed cellulose to glucose. | |||||||||||||||||
| 書誌情報 |
en : PLOS ONE 巻 13, 号 11, p. e0205915, 発行日 2018-11-09 |
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| 収録物識別子タイプ | ISSN | |||||||||||||||||
| 収録物識別子 | 19326203 | |||||||||||||||||
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| 出版者 | PLOS | |||||||||||||||||
| 権利情報 | ||||||||||||||||||
| 権利情報 | © 2018 Tsuji et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |||||||||||||||||
| EID | ||||||||||||||||||
| 識別子 | 347340 | |||||||||||||||||
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| 言語 | eng | |||||||||||||||||
