| Item type |
文献 / Documents(1) |
| 公開日 |
2019-01-29 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_db06 |
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資源タイプ |
doctoral thesis |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.3389/fendo.2017.00359 |
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関連名称 |
10.3389/fendo.2017.00359 |
| 出版タイプ |
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出版タイプ |
NA |
|
出版タイプResource |
http://purl.org/coar/version/c_be7fb7dd8ff6fe43 |
| タイトル |
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タイトル |
Effect of Osteocyte-Ablation on Inorganic Phosphate Metabolism : Analysis of Bone–Kidney–Gut Axis |
| タイトル別表記 |
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その他のタイトル |
骨細胞除去による無機リン代謝における影響 : 骨-腎臓-腸管連関の解析 |
| タイトル別表記 |
|
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その他のタイトル |
Phosphate Homeostasis and Osteocyte Ablation |
| 著者 |
藤井, 理
辰巳, 佐和子
オガタ, マオ
アラカキ, トモヒロ
サカグチ, ハルナ
ノムラ, ケンゴ
ミヤガワ, アツミ
イクタ, カヨ
ハナザキ, アイ
金子, 一郎
瀬川, 博子
宮本, 賢一
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| 抄録 |
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内容記述 |
In response to kidney damage, osteocytes increase the production of several hormones critically involved in mineral metabolism. Recent studies suggest that osteocyte function is altered very early in the course of chronic kidney disease. In the present study, to clarify the role of osteocytes and the canalicular network in mineral homeostasis, we performed four experiments. In Experiment 1, we investigated renal and intestinal Pi handling in osteocyte-less (OCL) model mice [transgenic mice with the dentin matrix protein-1 promoter-driven diphtheria toxin (DT)-receptor that were injected with DT]. In Experiment 2, we administered granulocyte colony-stimulating factor to mice to disrupt the osteocyte canalicular network. In Experiment 3, we investigated the role of osteocytes in dietary Pi signaling. In Experiment 4, we analyzed gene expression level fluctuations in the intestine and liver by comparing mice fed a high Pi diet and OCL mice. Together, the findings of these experiments indicate that osteocyte ablation caused rapid renal Pi excretion (P < 0.01) before the plasma fibroblast growth factor 23 (FGF23) and parathyroid hormone (PTH) levels increased. At the same time, we observed a rapid suppression of renal Klotho (P < 0.01), type II sodium phosphate transporters Npt2a (P < 0.01) and Npt2c (P < 0.05), and an increase in intestinal Npt2b (P < 0.01) protein. In OCL mice, Pi excretion in feces was markedly reduced (P < 0.01). Together, these effects of osteocyte ablation are predicted to markedly increase intestinal Pi absorption (P < 0.01), thus suggesting that increased intestinal Pi absorption stimulates renal Pi excretion in OCL mice. In addition, the ablation of osteocytes and feeding of a high Pi diet affected FGF15/bile acid metabolism and controlled Npt2b expression. In conclusion, OCL mice exhibited increased renal Pi excretion due to enhanced intestinal Pi absorption. We discuss the role of FGF23–Klotho on renal and intestinal Pi metabolism in OCL mice. |
| キーワード |
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主題 |
osteocyte |
| キーワード |
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主題 |
phosphate |
| キーワード |
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主題 |
fibroblast growth factor 23 |
| キーワード |
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主題 |
Klotho |
| キーワード |
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|
主題 |
intestine |
| キーワード |
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主題 |
kidney |
| キーワード |
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主題 |
liver |
| キーワード |
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主題 |
bile acid |
| 書誌情報 |
en : Frontiers in Endocrinology
巻 8,
p. 359,
発行日 2017-12-21
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
16642392 |
| 出版者 |
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出版者 |
Frontiers |
| 備考 |
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|
値 |
内容要旨・審査要旨・論文本文の公開
本論文は，著者Osamu Fujiiの学位論文として提出され，学位審査・授与の対象となっている。
学位授与者所属 : 徳島大学大学院栄養生命科学教育部（人間栄養科学専攻） |
| 権利情報 |
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権利情報 |
© 2017 Fujii, Tatsumi, Ogata, Arakaki, Sakaguchi, Nomura, Miyagawa, Ikuta, Hanazaki, Kaneko, Segawa and Miyamoto. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
| EID |
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識別子 |
342187 |
| 言語 |
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言語 |
eng |
| 報告番号 |
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学位授与番号 |
甲第3140号 |
| 学位記番号 |
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値 |
甲栄第249号 |
| 学位授与年月日 |
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学位授与年月日 |
2018-03-23 |
| 学位名 |
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学位名 |
博士（栄養学） |
| 学位授与機関 |
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学位授与機関名 |
徳島大学 |
k3140_abstract_review.pdf (309 KB)
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