| Item type |
文献 / Documents(1) |
| 公開日 |
2019-04-23 |
| アクセス権 |
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|
アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_db06 |
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資源タイプ |
doctoral thesis |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.1371/journal.pone.0209786 |
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関連名称 |
10.1371/journal.pone.0209786 |
| 出版タイプ |
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出版タイプ |
NA |
|
出版タイプResource |
http://purl.org/coar/version/c_be7fb7dd8ff6fe43 |
| タイトル |
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|
タイトル |
Blockade of platelet-derived growth factor receptor-β, not receptor-α ameliorates bleomycin-induced pulmonary fibrosis in mice |
| タイトル別表記 |
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その他のタイトル |
PDGFレセプターαではなくβの阻害がマウスにおけるブレオマイシン誘発肺線維症を改善する |
| タイトル別表記 |
|
|
その他のタイトル |
Role of platelet-derived growth factor receptor-α and -β in pulmonary fibrosis in mice |
| 著者 |
岸, 昌美
青野, 純典
佐藤, 正大
小山, 壱也
東, 桃代
アベ, シュウイチ
河野, 弘
岸, 潤
豊田, 優子
オカザキ, ヒロヤス
小川, 博久
上原, 久典
西岡, 安彦
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| 抄録 |
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内容記述 |
Platelet-derived growth factor (PDGF) has been implicated in the pathogenesis of pulmonary fibrosis. Nintedanib, a multi-kinase inhibitor that targets several tyrosine kinases, including PDGF receptor (PDGFR), was recently approved as an anti-fibrotic agent to reduce the deterioration of FVC in patients with idiopathic pulmonary fibrosis (IPF). However, the effects of PDGFR-α or -β on pulmonary fibrosis remain unclear. In an attempt to clarify their effects, we herein used blocking antibodies specific for PDGFR-α (APA5) and -β (APB5) in a bleomycin (BLM)-induced pulmonary fibrosis mouse model. The effects of these treatments on the growth of lung fibroblasts were examined using the 3H-thymidine incorporation assay in vitro. The anti-fibrotic effects of these antibodies were investigated with the Ashcroft score and collagen content of lungs treated with BLM. Their effects on inflammatory cells in the lungs were also analyzed using bronchoalveolar lavage fluid. We investigated damage to epithelial cells and the proliferation of fibroblasts in the lungs. APA5 and APB5 inhibited the phosphorylation of PDGFR-α and -β as well as the proliferation of lung fibroblasts induced by PDGF-AA and BB. The administration of APB5, but not APA5 effectively inhibited BLM-induced pulmonary fibrosis in mice. Apoptosis and the proliferation of epithelial cells and fibroblasts were significantly decreased by the treatment with APB5, but not by APA5. The late treatment with APB5 also ameliorated fibrosis in lungs treated with BLM. These results suggest that PDGFR-α and -β exert different effects on BLM-induced pulmonary fibrosis in mice. A specific approach using the blocking antibody for PDGFR-β may be useful for the treatment of pulmonary fibrosis. |
| キーワード |
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|
主題 |
Fibroblast |
| キーワード |
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|
主題 |
platelet-derived growth factor |
| キーワード |
|
|
主題 |
pulmonary fibrosis |
| 書誌情報 |
en : PLOS ONE
巻 13,
号 12,
p. e0209786,
発行日 2018-12-31
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
19326203 |
| 出版者 |
|
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出版者 |
PLOS |
| 備考 |
|
|
値 |
内容要旨・審査要旨・論文本文の公開
本論文は, 著者Masami Kishiの学位論文として提出され, 学位審査・授与の対象となっている。
学位授与者所属 : 徳島大学大学院医科学教育部（医学専攻） |
| 権利情報 |
|
|
権利情報 |
© 2018 Kishi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| EID |
|
|
識別子 |
351245 |
| 言語 |
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言語 |
eng |
| 報告番号 |
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学位授与番号 |
甲第3251号 |
| 学位記番号 |
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値 |
甲医第1400号 |
| 学位授与年月日 |
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学位授与年月日 |
2019-02-06 |
| 学位名 |
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学位名 |
博士（医学） |
| 学位授与機関 |
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|
学位授与機関名 |
徳島大学 |
k3251_abstract_review.pdf (230 KB)
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