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Targeting TNF-α suppresses the production of MMP-9 in human salivary gland cells
https://tokushima-u.repo.nii.ac.jp/records/2006608
https://tokushima-u.repo.nii.ac.jp/records/2006608ef6a2084-2b93-4dda-ada2-6c8a737b6d4d
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
archoralbio_58_12_1761.pdf (1.21 MB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||
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| 公開日 | 2019-08-23 | |||||||||||||||||
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| アクセス権 | open access | |||||||||||||||||
| 資源タイプ | ||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||
| 出版社版DOI | ||||||||||||||||||
| 関連識別子 | https://doi.org/10.1016/j.archoralbio.2013.09.004 | |||||||||||||||||
| 関連名称 | 10.1016/j.archoralbio.2013.09.004 | |||||||||||||||||
| 出版タイプ | ||||||||||||||||||
| 出版タイプ | AM | |||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||||||
| タイトル | ||||||||||||||||||
| タイトル | Targeting TNF-α suppresses the production of MMP-9 in human salivary gland cells | |||||||||||||||||
| 著者 |
青田, 桂子
× 青田, 桂子
WEKO
487
× 東, 雅之
WEKO
1711
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| 内容記述 | Objective: Tumor necrosis factor-α (TNF-α) is a pleiotropic cytokine that plays an essential role in inflammation and apoptosis. Our previous study suggested that TNF-α-induced activation of matrix metalloproteinase-9 (MMP-9) resulted in the destruction of acinar tissue in the salivary glands of patients with Sjögren’s syndrome (SS) via disruption of the acinar cell-basement membrane. Recently, a wide array of biological agents has been designed to inhibit TNF, including etanercept and adalimumab. In this study, we demonstrate the suppressive effect of anti-TNF agents on TNF-α-induced MMP-9 production in NS-AV-AC, an immortalized human salivary gland acinar cell line. Materials and Methods: NS-AV-AC cells were treated with etanercept or adalimumab after TNF-α treatment. MMP-9 production and enzymatic activity were, respectively, visualized by real-time PCR and ELISA assay, and evaluated by gelatin zymography, and apoptosis was evaluated by DNA fragmentation assay. Results: TNF-α induced the production of MMP-9 in NS-SV-AC cells. However, this production was greatly inhibited by treatment with etanercept or adalimumab. In addition, TNF-α-induced DNA fragmentation was prevented by treatment with etanercept or adalimumab. Conclusions: These results may indicate that anti-TNF agents would have therapeutic efficacy for preventing destruction of the acinar structure in the salivary glands of patients with SS. |
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| キーワード | ||||||||||||||||||
| 主題 | Salivary gland cells | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 主題 | Tumour necrosis factor-α | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 主題 | Matrix metalloproteinase-9 | |||||||||||||||||
| 書誌情報 |
en : Archives of Oral Biology 巻 58, 号 12, p. 1761-1768, 発行日 2013-09-21 |
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| 収録物識別子タイプ | ISSN | |||||||||||||||||
| 収録物識別子 | 00039969 | |||||||||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||||||||
| 収録物識別子 | AA00548457 | |||||||||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||||||||
| 収録物識別子 | AA11521918 | |||||||||||||||||
| 出版者 | ||||||||||||||||||
| 出版者 | Elsevier | |||||||||||||||||
| 権利情報 | ||||||||||||||||||
| 権利情報 | © 2013. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||||||||||||||
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| 識別子 | 284663 | |||||||||||||||||
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| 言語 | eng | |||||||||||||||||
