| Item type |
文献 / Documents(1) |
| 公開日 |
2019-08-23 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
関連識別子 |
https://doi.org/10.1186/s12943-018-0760-x |
|
|
関連名称 |
10.1186/s12943-018-0760-x |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
The microRNA-15a-PAI-2 axis in cholangiocarcinoma-associated fibroblasts promotes migration of cancer cells |
| 著者 |
Utaijaratrasmi, Penkhae
Vaeteewoottacharn, Kulthida
常松, 貴明
Jamjantra, Pranisa
Wongkham, Sopit
Pairojkul, Chawalit
Khuntikeo, Narong
石丸, 直澄
Sirivatanauksorn, Yongyut
Pongpaibul, Ananya
Thuwajit, Peti
Thuwajit, Chanitra
工藤, 保誠
|
| 抄録 |
|
|
内容記述 |
Background: Cholangiocarcinoma (CCA) has an abundance of tumor stroma which plays an important role in cancer progression via tumor-promoting signals. This study aims to explore the microRNA (miRNA) profile of CCA-associated fibroblasts (CCFs) and the roles of any identified miRNAs in CCA progression.
Methods: miRNA expression profiles of CCFs and normal skin fibroblasts were compared by microarray. Identified downregulated miRNAs and their target genes were confirmed by real-time PCR. Their binding was confirmed by a luciferase reporter assay. The effects of conditioned-media (CM) of miRNA mimic- and antagonist-transfected CCFs were tested in CCA migration in wound healing assays. Finally, the levels of miRNA and their target genes were examined by real-time PCR and immunohistochemistry in clinical CCA samples.
Results: miR-15a was identified as a downregulated miRNA in CCFs. Moreover, PAI-2 was identified as a novel target gene of miR-15a. Recombinant PAI-2 promoted migration of CCA cells. Moreover, CM from miR-15a mimic-transfected CCFs suppressed migration of CCA cells. Lower expression of miR-15a and higher expression of PAI-2 were observed in human CCA samples compared with normal liver tissues. Importantly, PAI-2 expression correlated with poor prognosis in CCA patients.
Conclusions: These findings highlight the miR-15a/PAI-2 axis as a potential therapeutic target in CCA patients. |
| キーワード |
|
|
主題 |
Cancer-associated fibroblasts |
| キーワード |
|
|
主題 |
microRNA (miRNA) |
| キーワード |
|
|
主題 |
Cholangiocarcinoma |
| キーワード |
|
|
主題 |
PAI-2 |
| キーワード |
|
|
主題 |
Migration |
| キーワード |
|
|
主題 |
Tumor microenvironment |
| 書誌情報 |
en : Molecular Cancer
巻 17,
p. 10,
発行日 2018-01-18
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
14764598 |
| 出版者 |
|
|
出版者 |
BioMed Central |
| 出版者 |
|
|
出版者 |
Springer Nature |
| 権利情報 |
|
|
権利情報 |
© The Author(s). 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
| EID |
|
|
識別子 |
335882 |
| 言語 |
|
|
言語 |
eng |
mc_17_10.pdf (4.23 MB)
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