| Item type |
文献 / Documents(1) |
| 公開日 |
2019-10-02 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1016/j.cell.2012.03.043 |
|
|
言語 |
ja |
|
|
関連名称 |
10.1016/j.cell.2012.03.043 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Cyclin F-Mediated Degradation of Ribonucleotide Reductase M2 Controls Genome Integrity and DNA Repair |
|
言語 |
en |
| 著者 |
D'Angiolella, Vincenzo
Donato, Valerio
Forrester, Frances M.
Jeong, Yeon-Tae
Pellacani, Claudia
工藤, 保誠
Saraf, Anita
Florens, Laurence
Washburn, Michael P.
Pagano, Michele
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
F-box proteins are the substrate binding subunits of SCF (Skp1-Cul1-F-box protein) ubiquitin ligase complexes. Using affinity purifications and mass spectrometry, we identified RRM2 (the ribonucleotide reductase family member 2) as an interactor of the F-box protein cyclin F. Ribonucleotide reductase (RNR) catalyzes the conversion of ribonucleotides to deoxyribonucleotides (dNTPs), which are necessary for both replicative and repair DNA synthesis. We found that, during G2, following CDK-mediated phosphorylation of Thr33, RRM2 is degraded via SCFcyclin F to maintain balanced dNTP pools and genome stability. After DNA damage, cyclin F is downregulated in an ATR-dependent manner to allow accumulation of RRM2. Defective elimination of cyclin F delays DNA repair and sensitizes cells to DNA damage, a phenotype that is reverted by expressing a nondegradable RRM2 mutant. In summary, we have identified a biochemical pathway that controls the abundance of dNTPs and ensures efficient DNA repair in response to genotoxic stress. |
|
言語 |
en |
| 書誌情報 |
en : Cell
巻 149,
号 5,
p. 1023-1034,
発行日 2012-05-25
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
00928674 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00600003 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12024453 |
| 出版者 |
|
|
出版者 |
Elsevier |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
Open Archive |
| EID |
|
|
識別子 |
248138 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
cell_149_5_1023.pdf (1.74 MB)
