| Item type |
文献 / Documents(1) |
| 公開日 |
2019-10-15 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.1016/j.jtemb.2016.01.011 |
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関連名称 |
10.1016/j.jtemb.2016.01.011 |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| タイトル |
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|
タイトル |
Iron-induced skeletal muscle atrophy involves an Akt-forkhead box O3–E3 ubiquitin ligase-dependent pathway |
| タイトル別表記 |
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その他のタイトル |
Iron-induced atrophy via Akt-FOXO3-E3 Ubiquitin ligase pathway |
| 著者 |
池田, 康将
イマオ, ミズキ
サトウ, アキホ
ワタナベ, ヒロアキ
濱野, 裕章
堀ノ内, 裕也
(井澤)石澤, 有紀
木平, 孝高
宮本, 理人
石澤, 啓介
土屋, 浩一郎
玉置, 俊晃
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| 抄録 |
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内容記述 |
Skeletal muscle wasting or sarcopenia is a critical health problem. Skeletal muscle atrophy is induced by an excess of iron, which is an essential trace metal for all living organisms. Excessive amounts of iron catalyze the formation of highly toxic hydroxyl radicals via the Fenton reaction. However, the molecular mechanism of iron-induced skeletal muscle atrophy has remained unclear. In this study, 8-weeks-old C57BL6/J mice were divided into 2 groups: vehicle-treated group and the iron-injected group (10 mg iron·day-1·mouse-1) during 2 weeks. Mice in the iron-injected group showed an increase in the iron content of the skeletal muscle and serum and ferritin levels in the muscle, along with reduced skeletal muscle mass. The skeletal muscle showed elevated mRNA expression of the muscle atrophy-related E3 ubiquitin ligases, atrogin-1 and muscle ring finger-1(MuRF1), on days 7 and 14 of iron treatment. Moreover, iron-treated mice showed reduced phosphorylation of Akt and forkhead box O3 (FOXO3a) in skeletal muscles. Inhibition of FOXO3a using siRNA in vitro in C2C12 myotube cells inhibited iron-induced upregulation of atrogin-1 and MuRF1 and reversed the reduction in myotube diameters. Iron-load caused oxidative stress, and an oxidative stress inhibitor abrogated iron-induced muscle atrophy by reactivating the Akt-FOXO3 pathway. Iron-induced skeletal muscle atrophy is suggested to involve the E3 ubiquitin ligase mediated by the reduction of Akt-FOXO3a signaling by oxidative stress. |
| キーワード |
|
|
主題 |
Iron |
| キーワード |
|
|
主題 |
Skeletal muscle atrophy |
| キーワード |
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|
主題 |
Atrogenes |
| 書誌情報 |
en : Journal of Trace Elements in Medicine and Biology
巻 35,
p. 66-76,
発行日 2016-01-28
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0946672X |
| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
18783252 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11067337 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12084444 |
| 出版者 |
|
|
出版者 |
Elsevier |
| 権利情報 |
|
|
権利情報 |
© 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| EID |
|
|
識別子 |
306466 |
| 言語 |
|
|
言語 |
eng |
jtemb_35_66.pdf (1.35 MB)
