WEKO3
-
RootNode
アイテム
In Situ Observation and Measurement of Actin Stress Fiber Deformation in Stretched Osteoblast like Cell
https://tokushima-u.repo.nii.ac.jp/records/2006899
https://tokushima-u.repo.nii.ac.jp/records/2006899d7ecdca9-5bb8-418b-a8d1-7c0497acaf4b
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
abb_8_11_421.pdf (3.51 MB)
|
.png)
|
| Item type | 文献 / Documents(1) | |||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2019-11-08 | |||||||||||||||||||||||||||||
| アクセス権 | ||||||||||||||||||||||||||||||
| アクセス権 | open access | |||||||||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||||||||||
| 出版社版DOI | ||||||||||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.4236/abb.2017.811031 | |||||||||||||||||||||||||||||
| 関連名称 | 10.4236/abb.2017.811031 | |||||||||||||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||||||||||
| タイトル | ||||||||||||||||||||||||||||||
| タイトル | In Situ Observation and Measurement of Actin Stress Fiber Deformation in Stretched Osteoblast like Cell | |||||||||||||||||||||||||||||
| 著者 |
佐藤, 克也
× 佐藤, 克也
WEKO
367
× ヌノビキ, ケンタ
× 藤澤, 正一郎× ナカハラ, タスク
× ミナミ, カズユキ
|
|||||||||||||||||||||||||||||
| 抄録 | ||||||||||||||||||||||||||||||
| 内容記述 | It is believed that mechanical stimuli, such as stretching of the extracellular matrix, are transmitted into cells via focal adhesion complexes and the actin cytoskeleton. Transmission dynamics of strain from the extracellular matrix into intracellular organelles is crucial to clarify the mechanosensing mechanisms of cells. In this study, we observed deformation behavior of actin stress fibers under uniaxial stretch using an originally developed cell-stretching microelectromechanical system (MEMS) device. It was difficult to conduct in situ observation of cells under stretch using conventional cell stretching devices, because motion artifacts such as rigid displacement during stretch application were not negligible. Our novel cell-stretching MEMS device suppressed rigid displacement while stretching, and we succeeded in obtaining time-lapse images of stretched cells. Uniaxial strain with a 10% magnitude and strain rate of 0.5%/sec was applied to cells. Deformation behaviors of the cells and actin stress fibers were recorded using a confocal laser scanning microscope. In time-lapse images of stretched cells, strains along each stress fiber were measured manually. As a result, in cells with a relatively homogeneous stress fiber structure oriented in one direction, distribution of the axial strain on stress fibers generally corresponded to deformation of the stretching sheet on which the cells had adhered. However, in cells with a heterogeneous stress fiber structure oriented in several directions, we found that the strain distribution along stress fibers was not homogeneous. In regions around the cell nucleus, there was a more complicated strain distribution compared with other regions. Our results suggest the cell nucleus with a stiff mechanical resistance yields such a complicated strain distribution in stress fibers. | |||||||||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||||||||
| 主題 | Actin Cytoskeleton | |||||||||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||||||||
| 主題 | Stretching Stimuli | |||||||||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||||||||
| 主題 | Osteoblast | |||||||||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||||||||
| 主題 | Cell Biomechanics | |||||||||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||||||||
| 主題 | MEMS | |||||||||||||||||||||||||||||
| 書誌情報 |
en : Advances in Bioscience and Biotechnology 巻 8, 号 11, p. 421-433, 発行日 2017-11-21 |
|||||||||||||||||||||||||||||
| 収録物ID | ||||||||||||||||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||||||||||||||||
| 収録物識別子 | 21568456 | |||||||||||||||||||||||||||||
| 収録物ID | ||||||||||||||||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||||||||||||||||
| 収録物識別子 | 21568502 | |||||||||||||||||||||||||||||
| 出版者 | ||||||||||||||||||||||||||||||
| 出版者 | Scientific Research Publishing | |||||||||||||||||||||||||||||
| 権利情報 | ||||||||||||||||||||||||||||||
| 権利情報 | © 2017 by authors and Scientific Research Publishing Inc. This work is licensed under the Creative Commons Attribution International License (CC BY 4.0). http://creativecommons.org/licenses/by/4.0/ | |||||||||||||||||||||||||||||
| EID | ||||||||||||||||||||||||||||||
| 識別子 | 335194 | |||||||||||||||||||||||||||||
| 言語 | ||||||||||||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||||||||||||
