| Item type |
文献 / Documents(1) |
| 公開日 |
2019-11-06 |
| アクセス権 |
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|
アクセス権 |
open access |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1007/s11010-015-2362-x |
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|
言語 |
ja |
|
|
関連名称 |
10.1007/s11010-015-2362-x |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| タイトル |
|
|
タイトル |
Effects of employment of distinct strategies to capture antibody on antibody delivery into cultured cells |
|
言語 |
en |
| 著者 |
クワハラ, カナ
ハラダ, カズキ
ヤマゴシ, リョウヘイ
山本, 武範
篠原, 康雄
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
The characteristics of antibody delivery into cultured HeLa cells were examined by using two delivery systems. Both systems used a cell-penetrating peptide as a tool for intrusion of an antibody into the cells, but either a “protein A derivative” or “hydrophobic motif” was employed to capture the antibody. When we examined the uptake of the Alexa Fluor-labeled antibody by use of these two systems, both systems were found to effectively deliver the antibody into the cultured cells. However, when we compared the amount of antibody delivered by these systems with the amount of transferrin uptake, the former was 10 times smaller than the latter. The lower efficiency of antibody delivery than transferrin uptake seemed to be attributable to the involvement of the antibody delivery reagent, which failed to catch the antibody molecule. This interpretation was validated by an experiment using a larger amount of antibody, and the amount of antibody delivered by the “protein A derivative” system under this condition was determined to be 13 ng proteins/105 cells. The antibody delivery achieved by the “protein A derivative” or “hydrophobic motif” showed two differences, i.e., a difference in intracellular distribution of the delivered antibody molecules and a difference in the fluorescence spectrum observed with cellular lysates. Possible reasons for these differences between the two delivery systems are discussed. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Antibody delivery |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Cell-penetrating peptide |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Protein A |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Hydrophobic motif |
| 書誌情報 |
en : Molecular and Cellular Biochemistry
巻 404,
号 1-2,
p. 25-30,
発行日 2015-02-20
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
03008177 |
| 収録物ID |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
15734919 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00745800 |
| 出版者 |
|
|
出版者 |
Springer US |
|
言語 |
en |
| 備考 |
|
|
言語 |
ja |
|
値 |
This is a post-peer-review, pre-copyedit version of an article published in Molecular and Cellular Biochemistry. The final authenticated version is available online at: https://doi.org/10.1007/s11010-015-2362-x. |
| EID |
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|
識別子 |
292705 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
mcb_404_1-2_25.pdf (780 KB)
