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Differential Regulation of Gene Expression of Alveolar Epithelial Cell Markers in Human Lung Adenocarcinoma-Derived A549 Clones
https://tokushima-u.repo.nii.ac.jp/records/2007149
https://tokushima-u.repo.nii.ac.jp/records/2007149dd46e885-d1d0-4df6-84b5-0de00bc40a1d
| 名前 / ファイル | ライセンス | アクション |
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sci_2015_165867.pdf (2.92 MB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||||||||||||||
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| 公開日 | 2020-02-04 | |||||||||||||||||||||||||||||
| アクセス権 | ||||||||||||||||||||||||||||||
| アクセス権 | open access | |||||||||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||||||||||
| 出版社版DOI | ||||||||||||||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1155/2015/165867 | |||||||||||||||||||||||||||||
| 言語 | ja | |||||||||||||||||||||||||||||
| 関連名称 | 10.1155/2015/165867 | |||||||||||||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||||||||||
| タイトル | ||||||||||||||||||||||||||||||
| タイトル | Differential Regulation of Gene Expression of Alveolar Epithelial Cell Markers in Human Lung Adenocarcinoma-Derived A549 Clones | |||||||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||||||
| 著者 |
コンドウ, ヒロシ
× コンドウ, ヒロシ
× 三好, 圭子
WEKO
1679
× 先山, 正二× 丹黒, 章
WEKO
176
× 野間, 隆文
WEKO
1658
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| 内容記述タイプ | Abstract | |||||||||||||||||||||||||||||
| 内容記述 | Stem cell therapy appears to be promising for restoring damaged or irreparable lung tissue. However, establishing a simple and reproducible protocol for preparing lung progenitor populations is difficult because the molecular basis for alveolar epithelial cell differentiation is not fully understood. We investigated an in vitro system to analyze the regulatory mechanisms of alveolus-specific gene expression using a human alveolar epithelial type II (ATII) cell line, A549. After cloning A549 subpopulations, each clone was classified into five groups according to cellmorphology andmarker gene expression. Two clones (B7 and H12)were further analyzed. Under serum-free culture conditions, surfactant protein C (SPC), an ATII marker, was upregulated in both H12 and B7. Aquaporin 5 (AQP5), an ATI marker, was upregulated in H12 and significantly induced in B7. When the RAS/MAPK pathway was inhibited, SPC and thyroid transcription factor-1 (TTF-1) expression levels were enhanced. After treatment with dexamethasone (DEX), 8-bromoadenosine 3’5’-cyclic monophosphate (8-Br-cAMP), 3-isobutyl-1-methylxanthine (IBMX), and keratinocyte growth factor (KGF), surfactant protein B and TTF-1 expression levels were enhanced. We found that A549-derived clones have plasticity in gene expression of alveolar epithelial differentiation markers and could be useful in studying ATII maintenance and differentiation. | |||||||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||||||
| 書誌情報 |
en : Stem Cells International 巻 2015, p. 165867, 発行日 2015-06-17 |
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| 収録物識別子タイプ | ISSN | |||||||||||||||||||||||||||||
| 収録物識別子 | 1687966X | |||||||||||||||||||||||||||||
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| 収録物識別子タイプ | ISSN | |||||||||||||||||||||||||||||
| 収録物識別子 | 16879678 | |||||||||||||||||||||||||||||
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| 出版者 | Hindawi | |||||||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||||||
| 権利情報 | ||||||||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||||||
| 権利情報 | © 2015 Hiroshi Kondo et al.This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | |||||||||||||||||||||||||||||
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| 識別子 | 308420 | |||||||||||||||||||||||||||||
| 識別子タイプ | URI | |||||||||||||||||||||||||||||
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| 言語 | eng | |||||||||||||||||||||||||||||
