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Differential Expression of Adenine Nucleotide Converting Enzymes in Mitochondrial Intermembrane Space : A Potential Role of Adenylate Kinase Isozyme 2 in Neutrophil Differentiation
https://tokushima-u.repo.nii.ac.jp/records/2007150
https://tokushima-u.repo.nii.ac.jp/records/20071503e86a757-ee8c-42e1-adc6-4ff4bdbfe0be
名前 / ファイル | ライセンス | アクション |
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Item type | 文献 / Documents(1) | |||||||||||||||||||||||||||||
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公開日 | 2020-02-04 | |||||||||||||||||||||||||||||
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アクセス権 | open access | |||||||||||||||||||||||||||||
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資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||||||||
資源タイプ | journal article | |||||||||||||||||||||||||||||
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関連識別子 | https://doi.org/10.1371/journal.pone.0089916 | |||||||||||||||||||||||||||||
関連名称 | 10.1371/journal.pone.0089916 | |||||||||||||||||||||||||||||
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出版タイプ | VoR | |||||||||||||||||||||||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||||||||||
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タイトル | Differential Expression of Adenine Nucleotide Converting Enzymes in Mitochondrial Intermembrane Space : A Potential Role of Adenylate Kinase Isozyme 2 in Neutrophil Differentiation | |||||||||||||||||||||||||||||
タイトル別表記 | ||||||||||||||||||||||||||||||
その他のタイトル | AK2 in Neutrophil Differentiation | |||||||||||||||||||||||||||||
著者 |
谷村, 綾子
× 谷村, 綾子× 堀口, 大吾
WEKO
508
× 三好, 圭子
WEKO
1679
× ハギタ, ヒロコ
× 野間, 隆文
WEKO
1658
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内容記述 | Adenine nucleotide dynamics in the mitochondrial intermembrane space (IMS) play a key role in oxidative phosphorylation. In a previous study, Drosophila adenylate kinase isozyme 2 (Dak2) knockout was reported to cause developmental lethality at the larval stage in Drosophila melanogaster. In addition, two other studies reported that AK2 is a responsible gene for reticular dysgenesis (RD), a human disease that is characterized by severe combined immunodeficiency and deafness. Therefore, mitochondrial AK2 may play an important role in hematopoietic differentiation and ontogenesis. Three additional adenine nucleotide metabolizing enzymes, including mitochondrial creatine kinases (CKMT1 and CKMT2) and nucleoside diphosphate kinase isoform D (NDPK-D), have been found in IMS. Although these kinases generate ADP for ATP synthesis, their involvement in RD remains unclear and still an open question. In this study, mRNA and protein expressions of these mitochondrial kinases were firstly examined in mouse ES cells, day 8 embryos, and 7-week-old adult mice. It was found that their expressions are spatiotemporally regulated, and Ak2 is exclusively expressed in bone marrow, which is a major hematopoietic tissue in adults. In subsequent experiments, we identified increased expression of both AK2 and CKMT1 during macrophage differentiation and exclusive production of AK2 during neutrophil differentiation using HL-60 cells as an in vitro model of hematopoietic differentiation. Furthermore, AK2 knockdown specifically inhibited neutrophil differentiation without affecting macrophage differentiation. These data suggest that AK2 is indispensable for neutrophil differentiation and indicate a possible causative link between AK2 deficiency and neutropenia in RD. | |||||||||||||||||||||||||||||
書誌情報 |
en : PLOS ONE 巻 9, 号 2, p. e89916, 発行日 2014-02-25 |
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収録物識別子タイプ | ISSN | |||||||||||||||||||||||||||||
収録物識別子 | 19326203 | |||||||||||||||||||||||||||||
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出版者 | PLOS | |||||||||||||||||||||||||||||
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権利情報 | © 2014 Tanimura et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |||||||||||||||||||||||||||||
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識別子 | 276026 | |||||||||||||||||||||||||||||
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言語 | eng |