| Item type |
文献 / Documents(1) |
| 公開日 |
2020-02-04 |
| アクセス権 |
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|
アクセス権 |
open access |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
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|
識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.22146/theindjdentres.10009 |
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言語 |
ja |
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|
関連名称 |
10.22146/theindjdentres.10009 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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|
タイトル |
Dynamic Changes of Sp6 Transgene Expression in Dental Epithelial Cells during Long-term Culture |
|
言語 |
en |
| 著者 |
Utami, Trianna W.
三好, 圭子
ハギタ, ヒロコ
Yanuaryska, Ryna D.
堀口, 大吾
野間, 隆文
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
To investigate the function of specificity protein 6 (SP6) transcription factor by gain-of-function procedure, we established cytomegalovirus (CMV) promoter-driven Sp6 stable transformants, C9 cells, using dental epithelial-derived cells. Initially, C9 cells produced a significant amount of SP6 protein. However, SP6 expression was reduced in these cells upon long-term culture. We could detect Sp6 transcripts in C9 cells by RT-PCR throughout the passages, although the CMV promoter is known to be epigenetically silenced. We recently found that SP6 was a short-lived protein that was degraded by a ubiquitin-independent proteasome pathway, although it is yet unclear how Sp6 expression was regulated during culture. Thus, we studied the possibility of epigenetic regulation of Sp6 expression. Comparative analysis of endogenous and exogenous Sp6 mRNA expressions demonstrated the specific down-regulation of exogenous Sp6 mRNA levels during culture passages. A DNA methyltransferase inhibitor, 5-Aza-2'-deoxycytidine (5AC), and a histone deacetylase inhibitor, valproic acid (VPA), enhanced or induced SP6 protein expression up to passage 28 without enhancing the mRNA level. The dramatic up-regulation of exogenous Sp6 mRNA was uniquely observed only at passage 50 by 5AC or VPA treatment. These findings indicate that multiple epigenetic regulatory mechanisms operate to fine-tune Sp6 expression during long-term culture. |
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言語 |
en |
| キーワード |
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言語 |
en |
|
主題Scheme |
Other |
|
主題 |
DNA methylation |
| キーワード |
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言語 |
en |
|
主題Scheme |
Other |
|
主題 |
epigenetic |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
regulation |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
gain-of-function |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
histone modification |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Sp6 |
| 書誌情報 |
en : The Indonesian Journal of Dental Research
巻 1,
号 3,
p. 134-142,
発行日 2011
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
20878710 |
| 収録物ID |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
24423300 |
| 出版者 |
|
|
出版者 |
Universitas Gadjah Mada |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License. |
| EID |
|
|
識別子 |
246861 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
indjdentres_1_3_134.pdf (1.05 MB)
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