| Item type |
文献 / Documents(1) |
| 公開日 |
2020-02-04 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1155/2011/320987 |
|
|
言語 |
ja |
|
|
関連名称 |
10.1155/2011/320987 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Possible Linkage of SP6 Transcriptional Activity with Amelogenesis by Protein Stabilization |
|
言語 |
en |
| 著者 |
Utami, Trianna W.
三好, 圭子
ハギタ, ヒロコ
Yanuaryska, Ryna Dwi
堀口, 大吾
野間, 隆文
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Ameloblasts produce enamel matrix proteins such as amelogenin, ameloblastin, and amelotin during tooth development. The molecular mechanisms of ameloblast differentiation (amelogenesis) are currently not well understood. SP6 is a transcription factor of the Sp/KLF family that was recently found to regulate cell proliferation in a cell-type-specific manner. Sp6-deficient mice demonstrate characteristic tooth anomalies such as delayed eruption of the incisors and supernumerary teeth with disorganized amelogenesis.However, it remains unclear how Sp6 controls amelogenesis. In this study, we used SP6 high producer cells to identify SP6 target genes. Based on the observations that long-term culture of SP6 high producer cells reduced SP6 protein expression but not Sp6 mRNA expression, we found that SP6 is short lived and specifically degraded through a proteasome pathway. We established an in vitro inducible SP6 expression system coupled with siRNA knockdown and found a possible linkage between SP6 and amelogenesis through the regulation of amelotin and Rock1 gene expression by microarray analysis. Our findings suggest that the regulation of SP6 protein stability is one of the crucial steps in amelogenesis. |
|
言語 |
en |
| 書誌情報 |
en : Journal of Biomedicine and Biotechnology
巻 2011,
p. 320987,
発行日 2011-10-20
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
11107243 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11698349 |
| 出版者 |
|
|
出版者 |
Hindawi |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
© 2011 TriannaW. Utami et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| EID |
|
|
識別子 |
242840 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
jbb_2011_320987.pdf (1.17 MB)
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