| Item type |
文献 / Documents(1) |
| 公開日 |
2020-02-05 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
https://doi.org/10.1016/S0925-4773(01)00549-4 |
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言語 |
ja |
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|
関連名称 |
10.1016/S0925-4773(01)00549-4 |
| 出版タイプ |
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|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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|
タイトル |
Conditional deletion of the bcl-x gene from mouse mammary epithelium results in accelerated apoptosis during involution but does not compromise cell function during lactation |
|
言語 |
en |
| 著者 |
Walton, Katherine D.
Wagner, Kay-Uwe
Rucker III, Edmund B.
Shillingford, Jonathan M.
三好, 圭子
Hennighausen, Lothar
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| 抄録 |
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|
内容記述タイプ |
Abstract |
|
内容記述 |
In the mammary gland Bcl-x is the most abundant cell survival factor from the Bcl-2 family. Since Bcl-x null mice die around day 12 of embryogenesis, the relevance of this protein in organ development and function is poorly understood. In erythroid cells bcl-x gene expression is controlled by cytokines and the transcription factor Stat5 (signal transducer and activator of transcription). However, we identified that bcl-x RNA levels in mammary tissue from prolactin receptor- and Stat5-null mice were indistinguishable from wild type mice. We have proposed that Bcl-x might control the survival of mammary epithelial cells throughout pregnancy, lactation, and the early stages of involution, and we have now tested this hypothesis through the conditional deletion of the bcl-x gene from mouse mammary epithelium. Conditional (floxed) bcl-x alleles were excised from alveolar cells during pregnancy using a Cre transgene under the control of the whey acidic protein gene promoter. Deletion of the bcl-x gene from the entire epithelial compartment (ducts and alveoli) was achieved by expressing Cre-recombinase under control of the mouse mammary tumor virus long terminal repeat. The absence of Bcl-x did not compromise proliferation and differentiation of mammary ductal and alveolar epithelial cells in virgin mice and during pregnancy and lactation. However, epithelial cell death and tissue remodeling were accelerated in the bcl-x conditional knockout mice during the first stage of involution. Concomitant deletion of the bax gene did not significantly modify the Bcl-x phenotype. Our results suggest that Bcl-x is not essential during mammopoiesis, but is critical for controlled apoptosis during the first phase of involution. Published by Elsevier Science Ireland Ltd. |
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言語 |
en |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Conditional gene deletion |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Mammary gland |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Epithelium |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Bcl-x |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Bax |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Apoptosis |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Involution |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Cre-recombinase |
| 書誌情報 |
en : Mechanisms of Development
巻 109,
号 2,
p. 281-293,
発行日 2001-09-27
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
09254773 |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10790903 |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11536507 |
| 出版者 |
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|
出版者 |
Elsevier |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
Open Archive |
| EID |
|
|
識別子 |
286330 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
md_109_2_281.pdf (5.88 MB)
