| Item type |
文献 / Documents(1) |
| 公開日 |
2020-03-13 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1371/journal.pntd.0004969 |
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言語 |
ja |
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|
関連名称 |
10.1371/journal.pntd.0004969 |
| 出版タイプ |
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|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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タイトル |
The Ectodomain of Glycoprotein from the Candid#1 Vaccine Strain of Junin Virus Rendered Machupo Virus Partially Attenuated in Mice Lacking IFN-αβ/γ Receptor |
|
言語 |
en |
| タイトル別表記 |
|
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その他のタイトル |
Contribution of GPC Ectodomain of Candid#1 |
|
言語 |
en |
| 著者 |
駒, 貴明
Huang, Cheng
Aronson, Judith F.
Walker, Aida G.
Miller, Milagros
Smith, Jeanon N.
Patterson, Michael
Paessler, Slobodan
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Machupo virus (MACV), a New World arenavirus, is the etiological agent of Bolivian hemorrhagic fever (BHF). Junin virus (JUNV), a close relative, causes Argentine hemorrhagic fever (AHF). Previously, we reported that a recombinant, chimeric MACV (rMACV/Cd#1-GPC) expressing glycoprotein from the Candid#1 (Cd#1) vaccine strain of JUNV is completely attenuated in a murine model and protects animals from lethal challenge with MACV. A rMACV with a single F438I substitution in the transmembrane domain (TMD) of GPC, which is equivalent to the F427I attenuating mutation in Cd#1 GPC, was attenuated in a murine model but genetically unstable. In addition, the TMD mutation alone was not sufficient to fully attenuate JUNV, indicating that other domains of the GPC may also contribute to the attenuation. To investigate the requirement of different domains of Cd#1 GPC for successful attenuation of MACV, we rescued several rMACVs expressing the ectodomain of GPC from Cd#1 either alone (MCg1), along with the TMD F438I substitution (MCg2), or with the TMD of Cd#1 (MCg3). All rMACVs exhibited similar growth curves in cultured cells. In mice, the MCg1 displayed significant reduction in lethality as compared with rMACV. The MCg1 was detected in brains and spleens of MCg1-infected mice and the infection was associated with tissue inflammation. On the other hand, all animals survived MCg2 and MCg3 infection without detectable levels of virus in various organs while producing neutralizing antibody against Cd#1. Overall our data suggest the indispensable role of each GPC domain in the full attenuation and immunogenicity of rMACV/Cd#1 GPC. |
|
言語 |
en |
| 書誌情報 |
en : PLOS Neglected Tropical Diseases
巻 10,
号 8,
p. e0004969,
発行日 2016-08-31
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
19352727 |
| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
19352735 |
| 出版者 |
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出版者 |
PLOS |
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言語 |
en |
| 権利情報 |
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|
言語 |
en |
|
権利情報 |
© 2016 Koma et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| EID |
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|
識別子 |
331132 |
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識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
pntd_10_8_e0004969.pdf (11 MB)
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