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RpoN Promotes Pseudomonas aeruginosa Survival in the Presence of Tobramycin
https://tokushima-u.repo.nii.ac.jp/records/2007368
https://tokushima-u.repo.nii.ac.jp/records/2007368ed68749a-6602-486e-a0ca-e7019d5bc4c9
| 名前 / ファイル | ライセンス | アクション |
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fmicb_8_839.pdf (1.74 MB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||||
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| 公開日 | 2020-11-19 | |||||||||||||||||||
| アクセス権 | ||||||||||||||||||||
| アクセス権 | open access | |||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||
| 出版社版DOI | ||||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||||
| 関連識別子 | https://doi.org/10.3389/fmicb.2017.00839 | |||||||||||||||||||
| 言語 | ja | |||||||||||||||||||
| 関連名称 | 10.3389/fmicb.2017.00839 | |||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||
| タイトル | ||||||||||||||||||||
| タイトル | RpoN Promotes Pseudomonas aeruginosa Survival in the Presence of Tobramycin | |||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| タイトル別表記 | ||||||||||||||||||||
| その他のタイトル | RpoN Mediates Tolerance to Tobramycin | |||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 著者 |
Viducic, Darija
× Viducic, Darija
× 村上, 圭史
WEKO
1687
× 天羽, 崇× (中西)小野, 恒子× 三宅, 洋一郎
WEKO
1651
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| 抄録 | ||||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||||
| 内容記述 | Pseudomonas aeruginosa has developed diverse strategies to respond and adapt to antibiotic stress. Among the factors that modulate survival in the presence of antibiotics, alternative sigma factors play an important role. Here, we demonstrate that the alternative sigma factor RpoN (σ 54) promotes survival in the presence of tobramycin. The tobramycin-sensitive phenotype of logarithmic phase ΔrpoN mutant cells is suppressed by the loss of the alternative sigma factor RpoS. Transcriptional analysis indicated that RpoN positively regulates the expression of RsmA, an RNA-binding protein, in the P. aeruginosa stationary growth phase in a nutrient-rich medium. The loss of RpoS led to the upregulation of gacA expression in the nutrient-limited medium-grown stationary phase cells. Conversely, in the logarithmic growth phase, the ΔrpoS mutant demonstrated lower expression of gacA, underscoring a regulatory role of RpoS for GacA. Supplementation of tobramycin to stationary phase ΔrpoN mutant cells grown in nutrient-rich medium resulted in decreased expression of gacA, relA, and rpoS without altering the expression of rsmA relative to wild-type PAO1. The observed downregulation of gacA and relA in the ΔrpoN mutant in the presence of tobramycin could be reversed through the mutation of rpoS in the ΔrpoN mutant background. The tobramycin-tolerant phenotype of the ΔrpoNΔrpoS mutant logarithmic phase cells may be associated with the expression of relA, which remained unresponsive upon addition of tobramycin. The logarithmic phase ΔrpoS and ΔrpoNΔrpoS mutant cells demonstrated increased expression of gacA in response to tobramycin. Together, these results suggest that a complex regulatory interaction between RpoN, RpoS, the Gac/Rsm pathway, and RelA modulates the P. aeruginosa response to tobramycin. | |||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| キーワード | ||||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||
| 主題 | Pseudomonas aeruginosa | |||||||||||||||||||
| キーワード | ||||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||
| 主題 | RpoN | |||||||||||||||||||
| キーワード | ||||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||
| 主題 | RpoS | |||||||||||||||||||
| キーワード | ||||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||
| 主題 | tobramycin | |||||||||||||||||||
| キーワード | ||||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||
| 主題 | antibiotic tolerance | |||||||||||||||||||
| 書誌情報 |
en : Frontiers in Microbiology 巻 8, p. 839, 発行日 2017-05-12 |
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| 収録物ID | ||||||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||||||
| 収録物識別子 | 1664302X | |||||||||||||||||||
| 出版者 | ||||||||||||||||||||
| 出版者 | Frontiers Media S.A. | |||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 権利情報 | ||||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 権利情報 | Copyright © 2017 Viducic, Murakami, Amoh, Ono and Miyake. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. | |||||||||||||||||||
| EID | ||||||||||||||||||||
| 識別子 | 327491 | |||||||||||||||||||
| 識別子タイプ | URI | |||||||||||||||||||
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| 言語 | eng | |||||||||||||||||||
