| Item type |
文献 / Documents(1) |
| 公開日 |
2020-11-19 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.3389/fmicb.2017.02542 |
|
|
言語 |
ja |
|
|
関連名称 |
10.3389/fmicb.2017.02542 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Production of HIV-1 vif mRNA Is Modulated by Natural Nucleotide Variations and SLSA1 RNA Structure in SA1D2prox Genomic Region |
|
言語 |
en |
| タイトル別表記 |
|
|
その他のタイトル |
Modulation of vif-mRNA by HIV-1-SA1D2prox |
|
言語 |
en |
| 著者 |
野間口, 雅子
土肥, 直哉
ヨシダ, トモヤ
駒, 貴明
足立, 隼
オオデ, ヒロタカ
イワタニ, ヤスマサ
ヨコヤマ, マサル
サトウ, ヒロノリ
足立, 昭夫
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Genomic RNA of HIV-1 contains localized structures critical for viral replication. Its structural analysis has demonstrated a stem-loop structure, SLSA1, in a nearby region of HIV-1 genomic splicing acceptor 1 (SA1). We have previously shown that the expression level of vif mRNA is considerably altered by some natural single-nucleotide variations (nSNVs) clustering in SLSA1 structure. In this study, besides eleven nSNVs previously identified by us, we totally found nine new nSNVs in the SLSA1-containing sequence from SA1, splicing donor 2, and through to the start codon of Vif that significantly affect the vif mRNA level, and designated the sequence SA1D2prox (142 nucleotides for HIV-1 NL4-3). We then examined by extensive variant and mutagenesis analyses how SA1D2prox sequence and SLSA1 secondary structure are related to vif mRNA level. While the secondary structure and stability of SLSA1 was largely changed by nSNVs and artificial mutations introduced to restore the original NL4-3 form from altered ones by nSNVs, no clear association of the two SLSA1 properties with vif mRNA level was observed. In contrast, when naturally occurring SA1D2prox sequences that contain multiple nSNVs were examined, we attained significant inverse correlation between the vif level and SLSA1 stability. These results may suggest that SA1D2prox sequence adapts over time, and also that the altered SA1D2prox sequence, SLSA1 stability, and vif level are mutually related. In total, we show here that the entire SA1D2prox sequence and SLSA1 stability critically contribute to the modulation of vif mRNA level. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
HIV-1 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
SA1 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
SLSA1 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
vif mRNA |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
nNSV |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
secondary RNA structure |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
SA1D2prox |
| 書誌情報 |
en : Frontiers in Microbiology
巻 8,
p. 2542,
発行日 2017-12-18
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1664302X |
| 出版者 |
|
|
出版者 |
Frontiers Media S.A. |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
Copyright © 2017 Nomaguchi, Doi, Yoshida, Koma, Adachi, Ode, Iwatani, Yokoyama, Sato and Adachi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
| EID |
|
|
識別子 |
341253 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
fmicb_8_2542.pdf (7.18 MB)
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