| Item type |
文献 / Documents(1) |
| 公開日 |
2020-11-19 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.3389/fmicb.2018.02510 |
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関連名称 |
10.3389/fmicb.2018.02510 |
| 出版タイプ |
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|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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|
タイトル |
CXCR4- and CCR5-Tropic HIV-1 Clones Are Both Tractable to Grow in Rhesus Macaques |
| タイトル別表記 |
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その他のタイトル |
HIV-1 Derivatives in Rhesus Macaques |
| 著者 |
土肥, 直哉
ミウラ, トモユキ
モリ, ヒロミ
サカワキ, ヒロミ
駒, 貴明
足立, 昭夫
野間口, 雅子
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| 抄録 |
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内容記述 |
A major issue for present HIV-1 research is to establish model systems that reflect or mimic viral replication and pathogenesis actually observed in infected humans. To this end, various strategies using macaques as infection targets have long been pursued. In particular, experimental infections of rhesus macaques by HIV-1 derivatives have been believed to be best suited, if practicable, for studies on interaction of HIV-1 and humans under various circumstances. Recently, through in vitro genetic manipulations and viral cell-adaptations, we have successfully generated a series of HIV-1 derivatives with CXCR4-tropism or CCR5-tropism that grow in macaque cells to various degrees. Of these viruses, those with best replicative potentials can grow comparably with a pathogenic SIVmac in macaque cells by counteracting major restriction factors TRIM5, APOBEC3, and tetherin proteins. In this study, rhesus macaques were challenged with CXCR4-tropic (MN4/LSDQgtu) or CCR5-tropic (gtu + A4CI1) virus. The two viruses were found to productively infect rhesus macaques, being rhesus macaque-tropic HIV-1 (HIV-1rmt). However, plasma viral RNA was reduced to be an undetectable level in infected macaques at 5–6 weeks post-infection and thereafter. While replicated similarly well in rhesus peripheral blood mononuclear cells, MN4/LSDQgtu grew much better than gtu + A4CI1 in the animals. To the best of our knowledge, this is the first report demonstrating that HIV-1 derivatives (variants) grow in rhesus macaques. These viruses certainly constitute firm bases for generating HIV-1rmt clones pathogenic for rhesus monkeys, albeit they grow more poorly than pathogenic SIVmac and SHIV clones reported to date. |
| キーワード |
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|
主題 |
HIV-1 |
| キーワード |
|
|
主題 |
primate model |
| キーワード |
|
|
主題 |
rhesus macaque |
| キーワード |
|
|
主題 |
HIV-1rmt |
| キーワード |
|
|
主題 |
CXCR4-tropic |
| キーワード |
|
|
主題 |
CCR5-tropic |
| 書誌情報 |
en : Frontiers in Microbiology
巻 9,
p. 2510,
発行日 2018-10-18
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1664302X |
| 出版者 |
|
|
出版者 |
Frontiers Media S.A. |
| 権利情報 |
|
|
権利情報 |
Copyright © 2018 Doi, Miura, Mori, Sakawaki, Koma, Adachi and Nomaguchi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
| EID |
|
|
識別子 |
353504 |
| 言語 |
|
|
言語 |
eng |
fmicb_9_2510.pdf (865 KB)
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