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Catecholamine Detection Using a Functionalized Poly(L-dopa)-Coated Gate Field-Effect Transistor
https://tokushima-u.repo.nii.ac.jp/records/2007418
https://tokushima-u.repo.nii.ac.jp/records/200741879e6da4d-d76e-41f6-a01d-4d2f1939cbe1
| 名前 / ファイル | ライセンス | アクション |
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acsomega_3_6_6719.pdf (2.49 MB)
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| Item type | 文献 / Documents(1) | |||||||||||||
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| 公開日 | 2022-07-04 | |||||||||||||
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| アクセス権 | open access | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| 資源タイプ | journal article | |||||||||||||
| 出版社版DOI | ||||||||||||||
| 識別子タイプ | DOI | |||||||||||||
| 関連識別子 | https://doi.org/10.1021/acsomega.8b00518 | |||||||||||||
| 言語 | ja | |||||||||||||
| 関連名称 | 10.1021/acsomega.8b00518 | |||||||||||||
| 出版タイプ | ||||||||||||||
| 出版タイプ | VoR | |||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Catecholamine Detection Using a Functionalized Poly(L-dopa)-Coated Gate Field-Effect Transistor | |||||||||||||
| 言語 | en | |||||||||||||
| 著者 |
加治佐, 平
× 加治佐, 平× Li, Wei
× ミチノブ, ツヨシ
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| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | A highly sensitive catecholamine (CA) sensor was created using a biointerface layer composed of a biopolymer and a potentiometric detection device. For the detection of CAs, 3-aminophenylboronic acid (3-NH2-PBA) was reacted with the carboxyl side chain of L-3,4-dihydroxyphenylalanine (L-dopa, LD) and the PBA-modified L-dopa was directly copolymerized with LD on an Au electrode, resulting in a 3.5 nm thick PBA-modified poly(PBA–LD/LD) layer-coated Au electrode. By connecting the PBA–LD-coated Au electrode to a field-effect transistor (FET), the molecular charge changes at the biointerface of the Au electrode, which was caused by di-ester binding of the PBA–CA complex, were transduced into gate surface potential changes. Effective CAs included LD, dopamine (DA), norepinephrine (NE), and epinephrine (EP). The surface potential of the PBA–LD-coated Au changed after the addition of 40 nM of each CA solution; notably, the PBA–LD-coated Au showed a higher sensitivity to LD because the surface potential change could already be observed after 1 nM of LD was added. The fundamental parameter analyses of the PBA–LD to CA affinity from the surface potential shift against each CA concentration indicated the highest affinity to LD (binding constant (Ks): 1.68 × 106 M–1, maximum surface potential shift (Vmax): 182 mV). Moreover, the limit of detection for each CA was 3.5 nM in LD, 12.0 nM in DA, 7.5 nM in NE, and 12.6 nM in EP. From these results, it is concluded that the poly(PBA–LD/LD)-coated gate FET could become a useful biosensor for neurotransmitters, hormones, and early detection of Parkinson’s disease. | |||||||||||||
| 言語 | en | |||||||||||||
| 書誌情報 |
en : ACS Omega 巻 3, 号 6, p. 6719-6727, 発行日 2018-06-20 |
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| 収録物識別子タイプ | ISSN | |||||||||||||
| 収録物識別子 | 24701343 | |||||||||||||
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| 出版者 | ACS Publications | |||||||||||||
| 言語 | en | |||||||||||||
| 権利情報 | ||||||||||||||
| 言語 | en | |||||||||||||
| 権利情報 | This is an open access article published under an ACS AuthorChoice License (https://pubs.acs.org/page/policy/authorchoice_termsofuse.html), which permits copying and redistribution of the article or any adaptations for non-commercial purposes. | |||||||||||||
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| 識別子 | 363922 | |||||||||||||
| 識別子タイプ | URI | |||||||||||||
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| 言語 | eng | |||||||||||||
