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Biocompatible Poly(catecholamine)-Film Electrode for Potentiometric Cell Sensing
https://tokushima-u.repo.nii.ac.jp/records/2007419
https://tokushima-u.repo.nii.ac.jp/records/20074192bd8cbe7-179d-4a96-bdce-1893f2c7928a
| 名前 / ファイル | ライセンス | アクション |
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acssensors_3_2_476.pdf (1.01 MB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||||||||
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| 公開日 | 2020-04-30 | |||||||||||||||||||||||
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| アクセス権 | open access | |||||||||||||||||||||||
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| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||||
| 出版社版DOI | ||||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1021/acssensors.7b00897 | |||||||||||||||||||||||
| 関連名称 | 10.1021/acssensors.7b00897 | |||||||||||||||||||||||
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| 出版タイプ | AM | |||||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||||||||||||
| タイトル | ||||||||||||||||||||||||
| タイトル | Biocompatible Poly(catecholamine)-Film Electrode for Potentiometric Cell Sensing | |||||||||||||||||||||||
| 著者 |
加治佐, 平
× 加治佐, 平× ヤナギモト, ヨシユキ
× サイトウ, アキコ
× サカタ, トシヤ
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| 内容記述 | Surface-coated poly(catecholamine) (pCA) films have attracted attention as biomaterial interfaces owing to their biocompatible and physicochemical characteristics. In this paper, we report that pCA-film-coated electrodes are useful for potentiometric biosensing devices. Four different types of pCA film—L-dopa, dopamine, norepinephrine, and epinephrine—with thicknesses in the range of 7–27 nm were electropolymerized by oxidation on Au electrodes by using cyclic voltammetry. By using the pCA-film electrodes, the pH responsivities were found to be 39.3 to 47.7 mV/pH within the pH range of 1.68 to 10.01 on the basis of the equilibrium reaction with hydrogen ions and the functional groups of the pCAs. The pCA films suppressed nonspecific signals generated by other ions (Na+, K+, Ca2+) and proteins such as albumin. Thus, the pCA-film electrodes can be used in pH-sensitive and pH-selective biosensors. HeLa cells were cultivated on the surface of the pCA-film electrodes to monitor cellular activities. The surface potential of the pCA-film electrodes changed markedly because of cellular activity; therefore, the change in the hydrogen ion concentration around the cell/pCA-film interface could be monitored in real time. This was caused by carbon dioxide or lactic acid that is generated by cellular respiration and dissolves in the culture medium, resulting in the change of hydrogen concentration. pCA-film electrodes are suitable for use in biocompatible and pH-responsive biosensors, enabling the more selective detection of biological phenomena. | |||||||||||||||||||||||
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| 主題 | biomimetic material | |||||||||||||||||||||||
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| 主題 | poly(catecholamine) | |||||||||||||||||||||||
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| 主題 | pH-sensitive biosensor | |||||||||||||||||||||||
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| 主題 | voltage follower circuit | |||||||||||||||||||||||
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| 主題 | cell sensing | |||||||||||||||||||||||
| 書誌情報 |
en : ACS Sensors 巻 3, 号 2, p. 476-483, 発行日 2018-01-23 |
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| 収録物識別子タイプ | ISSN | |||||||||||||||||||||||
| 収録物識別子 | 23793694 | |||||||||||||||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||||||||||||||
| 収録物識別子 | AA12774646 | |||||||||||||||||||||||
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| 出版者 | ACS Publications | |||||||||||||||||||||||
| 権利情報 | ||||||||||||||||||||||||
| 権利情報 | This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Sensors, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acssensors.7b00897. | |||||||||||||||||||||||
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| 識別子 | 363923 | |||||||||||||||||||||||
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| 言語 | eng | |||||||||||||||||||||||
