| Item type |
文献 / Documents(1) |
| 公開日 |
2020-04-16 |
| アクセス権 |
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|
アクセス権 |
open access |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_db06 |
|
資源タイプ |
doctoral thesis |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.1038/s41598-019-49232-8 |
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関連名称 |
10.1038/s41598-019-49232-8 |
| 出版タイプ |
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|
出版タイプ |
NA |
|
出版タイプResource |
http://purl.org/coar/version/c_be7fb7dd8ff6fe43 |
| タイトル |
|
|
タイトル |
Actin Cytoskeletal Reorganization Function of JRAB/MICAL-L2 Is Fine-tuned by Intramolecular Interaction between First LIM Zinc Finger and C-terminal Coiled-coil Domains |
| タイトル別表記 |
|
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その他のタイトル |
分子内結合が調節するJRABのLIMドメインによるアクチン細胞骨格の再編成 |
| 著者 |
三宅, 一央
坂根, 亜由子
ツチヤ, ユウコ
佐川, 幾子
トミダ, ヨウコ
笠原, 二郎
井本, 逸勢
ワタナベ, シオ
ヒゴ, ダイスケ
ミズグチ, ケンジ
佐々木, 卓也
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| 抄録 |
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内容記述 |
JRAB/MICAL-L2 is an effector protein of Rab13, a member of the Rab family of small GTPase. JRAB/MICAL-L2 consists of a calponin homology domain, a LIM domain, and a coiled-coil domain. JRAB/MICAL-L2 engages in intramolecular interaction between the N-terminal LIM domain and the C-terminal coiled-coil domain, and changes its conformation from closed to open under the effect of Rab13. Open-form JRAB/MICAL-L2 induces the formation of peripheral ruffles via an interaction between its calponin homology domain and filamin. Here, we report that the LIM domain, independent of the C-terminus, is also necessary for the function of open-form JRAB/MICAL-L2. In mechanistic terms, two zinc finger domains within the LIM domain bind the first and second molecules of actin at the minus end, potentially inhibiting the depolymerization of actin filaments (F-actin). The first zinc finger domain also contributes to the intramolecular interaction of JRAB/MICAL-L2. Moreover, the residues of the first zinc finger domain that are responsible for the intramolecular interaction are also involved in the association with F-actin. Together, our findings show that the function of open-form JRAB/MICAL-L2 mediated by the LIM domain is fine-tuned by the intramolecular interaction between the first zinc finger domain and the C-terminal domain. |
| キーワード |
|
|
主題 |
JRAB/MICAL-L2 |
| キーワード |
|
|
主題 |
Actin Cytoskeleton |
| キーワード |
|
|
主題 |
Intramolecular interaction |
| キーワード |
|
|
主題 |
LIM domain |
| 書誌情報 |
en : Scientific Reports
巻 9,
p. 12794,
発行日 2019-09-05
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
20452322 |
| 出版者 |
|
|
出版者 |
Springer Nature |
| 備考 |
|
|
値 |
内容要旨・審査要旨・論文本文の公開
本論文は，著者Kazuhisa Miyakeの学位論文として提出され，学位審査・授与の対象となっている。
学位授与者所属 : 徳島大学大学院医科学教育部（医学専攻） |
| 権利情報 |
|
|
権利情報 |
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| EID |
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識別子 |
370525 |
| 言語 |
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|
言語 |
eng |
| 報告番号 |
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|
学位授与番号 |
甲第3359号 |
| 学位記番号 |
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|
値 |
甲医第1442号 |
| 学位授与年月日 |
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|
学位授与年月日 |
2020-02-27 |
| 学位名 |
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学位名 |
博士（医学） |
| 学位授与機関 |
|
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|
学位授与機関名 |
徳島大学 |
k3359_abstract_review.pdf (241 KB)
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