| Item type |
文献 / Documents(1) |
| 公開日 |
2020-05-14 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_db06 |
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資源タイプ |
doctoral thesis |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.1038/s41598-019-51381-9 |
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関連名称 |
10.1038/s41598-019-51381-9 |
| 出版タイプ |
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出版タイプ |
NA |
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出版タイプResource |
http://purl.org/coar/version/c_be7fb7dd8ff6fe43 |
| タイトル |
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タイトル |
Coordination of WNT signaling and ciliogenesis during odontogenesis by piezo type mechanosensitive ion channel component 1 |
| タイトル別表記 |
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その他のタイトル |
ピエゾ型機械受容チャネル1による歯の発生過程におけるWNTシグナルと一次繊毛発現の調整 |
| 著者 |
宮嵜, 彩
杉本, 明日菜
ヨシザキ, ケイゴ
河原林, 啓太
イワタ, ココロ
クロゴウシ, リカ
北村, 尚正
大塚, 邦紘
長谷川, 智一
赤澤, 友基
フクモト, サトシ
石丸, 直澄
岩本, 勉
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| 抄録 |
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内容記述 |
Signal transmission from the mechanical forces to the various intracellular activities is a fundamental process during tissue development. Despite their critical role, the mechanism of mechanical forces in the biological process is poorly understood. In this study, we demonstrated that in the response to hydrostatic pressure (HP), the piezo type mechanosensitive ion channel component 1 (PIEZO1) is a primary mechanosensing receptor for odontoblast differentiation through coordination of the WNT expression and ciliogenesis. In stem cells from human exfoliated deciduous teeth (SHED), HP significantly promoted calcium deposition as well as the expression of odontogenic marker genes, PANX3 and DSPP, and WNT related-genes including WNT5b and WNT16, whereas HP inhibited cell proliferation and enhanced primary cilia expression. WNT signaling inhibitor XAV939 and primary cilia inhibitor chloral hydrate blocked the HP-induced calcium deposition. The PIEZO1 activator Yoda1 inhibited cell proliferation but induced ciliogenesis and WNT16 expression. Interestingly, HP and Yoda1 promoted nuclear translocation of RUNX2, whereas siRNA-mediated silencing of PIEZO1 decreased HP-induced nuclear translocation of RUNX2. Taken together, these results suggest that PIEZO1 functions as a mechanotransducer that connects HP signal to the intracellular signalings during odontoblast differentiation. |
| キーワード |
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主題 |
PIEZO1 |
| キーワード |
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|
主題 |
WNT |
| キーワード |
|
|
主題 |
hydrostatic pressure |
| キーワード |
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|
主題 |
odontblast |
| キーワード |
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|
主題 |
RUNX2 |
| 書誌情報 |
en : Scientific Reports
巻 9,
p. 14762,
発行日 2019-10-14
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
20452322 |
| 出版者 |
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出版者 |
Springer Nature |
| 備考 |
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|
値 |
内容要旨・審査要旨・論文本文の公開
本論文は, 著者Aya Miyazakiの学位論文として提出され, 学位審査・授与の対象となっている。
学位授与者所属 : 徳島大学大学院口腔科学教育部（口腔科学専攻） |
| 権利情報 |
|
|
権利情報 |
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
© The Author(s) 2019 |
| EID |
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識別子 |
359980 |
| 言語 |
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言語 |
eng |
| 報告番号 |
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学位授与番号 |
甲第3373号 |
| 学位記番号 |
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値 |
甲口第451号 |
| 学位授与年月日 |
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学位授与年月日 |
2020-03-23 |
| 学位名 |
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学位名 |
博士（歯学） |
| 学位授与機関 |
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学位授与機関名 |
徳島大学 |
k3373_abstract.pdf (137 KB)
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