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Advanced glycation end-products increase lipocalin 2 expression in human oral epithelial cells
https://tokushima-u.repo.nii.ac.jp/records/2007614
https://tokushima-u.repo.nii.ac.jp/records/20076148555d19d-c9c8-45a3-92a6-36ddafd58a78
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k3424_abstract.pdf (175 KB)
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k3424_review.pdf (59.2 KB)
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k3424_fulltext.pdf (980 KB)
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| Item type | 文献 / Documents(1) | |||||||||||
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| 公開日 | 2020-05-20 | |||||||||||
| アクセス権 | ||||||||||||
| アクセス権 | open access | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||||
| 資源タイプ | doctoral thesis | |||||||||||
| 出版社版DOI | ||||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | https://doi.org/10.1111/jre.12741 | |||||||||||
| 言語 | ja | |||||||||||
| 関連名称 | 10.1111/jre.12741 | |||||||||||
| 出版タイプ | ||||||||||||
| 出版タイプ | NA | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_be7fb7dd8ff6fe43 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Advanced glycation end-products increase lipocalin 2 expression in human oral epithelial cells | |||||||||||
| 言語 | en | |||||||||||
| タイトル別表記 | ||||||||||||
| その他のタイトル | 最終糖化産物は口腔上皮細胞におけるLipocain2の発現を増加させる | |||||||||||
| 言語 | ja | |||||||||||
| タイトル別表記 | ||||||||||||
| その他のタイトル | AGEs increase lipocalin 2 expression | |||||||||||
| 言語 | en | |||||||||||
| 著者 |
木戸, 理恵
× 木戸, 理恵× 廣島, 佑香× 木戸, 淳一× 生田, 貴久× 坂本, 英次郎
× 稲垣, 裕司× 成石, 浩司× 湯本, 浩通 |
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| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | Background and Objectives: Diabetes mellitus (DM), a risk factor of periodontal diseases, exacerbates the pathological condition of periodontitis. A major factor for DM complications is advanced glycation end-products (AGEs) that accumulate in periodontal tissues and cause inflammatory events. Lipocalin 2 (LCN2) is an antimicrobial peptide and inflammation-related factor, and LCN2 levels increase in DM. In the present study, the effects of AGEs and lipopolysaccharide of Porphyromonas gingivalis (P.g-LPS) on LCN2 expression in human oral epithelial cells (TR146 cells) and the role of secreted LCN2 in periodontitis with DM were investigated. Material and Methods: TR146 cells were cultured with AGEs (AGE2) and control BSA and cell viability was estimated, or with P.g-LPS. Conditioned medium and cell lysates were prepared from cultures of epithelial cells and used for western blotting and ELISA to analyze LCN2, RAGE, IL-6, MAPK and NF-κB. RNA was isolated from AGE-treated TR146 cells and differentiated HL-60 (D-HL-60) cells and used for quantitative real-time PCR to examine the expression of LCN2 and interleukin-6 (IL-6) mRNAs. RAGE- and LCN2-siRNAs (siRAGE, siLCN2) were transfected into epithelial cells, and AGE-induced LCN2 expression was investigated. D-HL-60 cells were co-cultured with TR146 cells that were transfected with siLCN2 and treated with AGEs, IL-6 mRNA expression in D-HL-60 cells and cell migration were investigated. Results: AGEs increased the expression levels of LCN2 and IL-6 in oral epithelial cells. siRAGE and a neutralizing antibody for RAGE inhibited AGE-induced LCN2 expression. AGEs stimulated the phosphorylation of ERK, p38 and NF-kB in epithelial cells, and their inhibitors suppressed AGE-induced LCN2 expression. In contrast, P.g-LPS did not show a significant increase on LCN2 level in TR146 cells that expressed toll-like receptor 2. In co-culture experiments, AGE-induced LCN2 inhibited IL-6 mRNA expression in D-HL-60 cells, and LCN2 knockdown in epithelial cells suppressed HL-60 cell migration. Conclusion: These results suggested that AGEs increase LCN2 expression via RAGE, MAPK, and NF-κB signaling pathways in oral epithelial cells, and secreted LCN2 may influence the pathological condition of periodontitis with DM. |
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| 言語 | en | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | AGEs | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | lipocalin2 | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | diabetes mellitus | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | periodontitis | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | human oral epithelial cells | |||||||||||
| 書誌情報 |
en : Journal of Periodontal Research 巻 55, 号 4, p. 539-550, 発行日 2020-03-13 |
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| 収録物ID | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 00223484 | |||||||||||
| 収録物ID | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 16000765 | |||||||||||
| 収録物ID | ||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||
| 収録物識別子 | AA00704381 | |||||||||||
| 収録物ID | ||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||
| 収録物識別子 | AA11628616 | |||||||||||
| 出版者 | ||||||||||||
| 出版者 | Wiley | |||||||||||
| 言語 | en | |||||||||||
| 備考 | ||||||||||||
| 言語 | ja | |||||||||||
| 値 | 内容要旨・審査要旨・論文本文の公開 本論文は，著者Rie Kidoの学位論文として提出され，学位審査・授与の対象となっている。 This is the peer reviewed version of the following article: Kido, R, Hiroshima, Y, Kido, J‐I, et al. Advanced glycation end‐products increase lipocalin 2 expression in human oral epithelial cells. J Periodont Res. 2020; 55: 539– 550, which has been published in final form at https://doi.org/10.1111/jre.12741. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. 学位授与者所属 : 徳島大学大学院口腔科学教育部（口腔科学専攻） |
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| EID | ||||||||||||
| 識別子 | 363586 | |||||||||||
| 識別子タイプ | URI | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| 報告番号 | ||||||||||||
| 学位授与番号 | 甲第3424号 | |||||||||||
| 学位記番号 | ||||||||||||
| 言語 | ja | |||||||||||
| 値 | 甲口第462号 | |||||||||||
| 学位授与年月日 | ||||||||||||
| 学位授与年月日 | 2020-04-09 | |||||||||||
| 学位名 | ||||||||||||
| 言語 | ja | |||||||||||
| 学位名 | 博士（歯学） | |||||||||||
| 学位授与機関 | ||||||||||||
| 言語 | ja | |||||||||||
| 学位授与機関名 | 徳島大学 | |||||||||||
