| Item type |
文献 / Documents(1) |
| 公開日 |
2020-05-28 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1016/j.ijpharm.2019.119010 |
|
|
言語 |
ja |
|
|
関連名称 |
10.1016/j.ijpharm.2019.119010 |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| タイトル |
|
|
タイトル |
Characteristics of unique endocytosis induced by weak current for cytoplasmic drug delivery |
|
言語 |
en |
| 著者 |
トラオ, タスク
ミムラ, ミユキ
オオシマ, ヤスフミ
フジカワ, コウキ
Hasan, Mahadi
シモカワ, タツハル
山﨑, 尚志
安藤, 英紀
石田, 竜弘
福田, 達也
田中, 保
小暮, 健太朗
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| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
We previously reported that 20 a weak current (WC, 0.3-0.5mA/cm2) applied to cells can induce endocytosis to promote cytoplasmic delivery of hydrophilic macromolecules (MW: < 70,000), such as dextran and siRNA, which leak from WC-induced endosomes into the cytoplasm (Hasan et al., 2016). In this study, we evaluated the characteristics of WC-mediated endocytosis for application of the technology to cytoplasmic delivery of macromolecular medicines. WC induced significantly higher cellular uptake of exogenous DNA fragments compared to untreated cells; the amount increased in a time-dependent manner, indicating that endocytosis was induced after WC. Moreover, following WC treatment of cells in the presence of an antibody (MW: 150,000) with the lysosomotropic agent chloroquine, the antibody was able to bind to its intracellular target. Thus, high molecular weight protein medicines delivered by WC-mediated endocytosis were functional in the cytoplasm. Transmission electron microscopy of cells treated by WC in the presence of gold nanoparticles covered with polyethylene glycol showed that the WC-induced endosomes exhibited an elliptical shape. In the WC-induced endosomes, ceramide, which makes pore structures in the membrane, was localized. Together, these results suggest that WC can induce unique endocytosis and that macromolecular medicines leak from endosomes through a ceramide pore. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Weak current |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Cellular uptake |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Cytoplasmic delivery |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Endosomal leakiness |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Ceramide pore |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Tubular endocytosis |
| 書誌情報 |
en : International Journal of Pharmaceutics
巻 576,
p. 119010,
発行日 2019-12-31
|
| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
03785173 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00680771 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11530828 |
| 出版者 |
|
|
出版者 |
Elsevier |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
© 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| EID |
|
|
識別子 |
362596 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
ijpharm_576_119010.pdf (2.22 MB)
