| Item type |
文献 / Documents(1) |
| 公開日 |
2020-06-15 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1242/bio.048728 |
|
|
言語 |
ja |
|
|
関連名称 |
10.1242/bio.048728 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
The absence of SOX2 in the anterior foregut alters the esophagus into trachea and bronchi in both epithelial and mesenchymal components |
|
言語 |
en |
| 著者 |
テラモト, マチコ
スガワラ, リョウ
ミネギシ, カツラ
ウチカワ, マサノリ
竹本, 龍也
クロイワ, アツシ
イシイ, ヤスオ
コンドウ, ヒサト
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
In the anterior foregut (AFG) of mouse embryos, the transcription factor SOX2 is expressed in the epithelia of the esophagus and proximal branches of respiratory organs comprising the trachea and bronchi, whereas NKX2.1 is expressed only in the epithelia of respiratory organs. Previous studies using hypomorphic Sox2 alleles have indicated that reduced SOX2 expression causes the esophageal epithelium to display some respiratory organ characteristics. In the present study, we produced mouse embryos with AFG-specific SOX2 deficiency. In the absence of SOX2 expression, a single NKX2.1-expressing epithelial tube connected the pharynx and the stomach, and a pair of bronchi developed in the middle of the tube. Expression patterns of NKX2.1 and SOX9 revealed that the anterior and posterior halves of SOX2-deficient AFG epithelial tubes assumed the characteristics of the trachea and bronchus, respectively. In addition, we found that mesenchymal tissues surrounding the SOX2-deficient NKX2.1-expressing epithelial tube changed to those surrounding the trachea and bronchi in the anterior and posterior halves, as indicated by the arrangement of smooth muscle cells and SOX9-expressing cells and by the expression of Wnt4 (esophagus specific), Tbx4 (respiratory organ specific), and Hoxb6 (distal bronchus specific). The impact of mesenchyme-derived signaling on the early stage of AFG epithelial specification has been indicated. Our study demonstrated an opposite trend where epithelial tissue specification causes concordant changes in mesenchymal tissues, indicating a reciprocity of epithelial-mesenchymal interactions. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Anterior foregut |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Conditional knockout |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Epithelial-mesenchymal interaction |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Esophagus |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Respiratory organ |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
SOX2 |
| 書誌情報 |
en : Biology Open
巻 9,
号 2,
p. bio048728,
発行日 2020-02-07
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
20466390 |
| 出版者 |
|
|
出版者 |
The Company of Biologists |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
| EID |
|
|
識別子 |
365128 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
bio_9_2_bio048728.pdf (15.4 MB)
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