| Item type |
文献 / Documents(1) |
| 公開日 |
2020-06-15 |
| アクセス権 |
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|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1186/s12860-015-0054-8 |
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|
言語 |
ja |
|
|
関連名称 |
10.1186/s12860-015-0054-8 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
A novel myogenic function residing in the 5′ non-coding region of Insulin receptor substrate-1 (Irs-1) transcript |
|
言語 |
en |
| 著者 |
ナガノ, ヒカル
ヤマギシ, ナオコ
トミダ, チサト
ヤノ, チアキ
アイバラ, カナ
コウノ, ショウヘイ
安倍, 知紀
真板, 綾子
平坂, 勝也
奥村, 裕司
Mills, Edward M
二川, 健
(手嶋)近藤, 茂忠
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Background: There is evidence that several messenger RNAs (mRNAs) are bifunctional RNAs, i.e. RNA transcript carrying both protein-coding capacity and activity as functional non-coding RNA via 5′ and 3′ untranslated regions (UTRs).
Results: In this study, we identified a novel bifunctional RNA that is transcribed from insulin receptor substrate-1 (Irs-1) gene with full-length 5′UTR sequence (FL-Irs-1 mRNA). FL-Irs-1 mRNA was highly expressed only in skeletal muscle tissue. In cultured skeletal muscle C2C12 cells, the FL-Irs-1 transcript functioned as a bifunctional mRNA. The FL-Irs-1 transcript produced IRS-1 protein during differentiation of myoblasts into myotubes; however, this transcript functioned as a regulatory RNA in proliferating myoblasts. The FL-Irs-1 5′UTR contains a partial complementary sequence to Rb mRNA, which is a critical factor for myogenic differentiation. The overexpression of the 5′UTR markedly reduced Rb mRNA expression, and this reduction was fully dependent on the complementary element and was not compensated by IRS-1 protein. Conversely, knockdown of FL-Irs-1 mRNA increased Rb mRNA expression and enhanced myoblast differentiation into myotubes.
Conclusions: Our findings suggest that the FL-Irs-1 transcript regulates myogenic differentiation as a regulatory RNA in myoblasts. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Bifunctional RNA |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Insulin receptor substrate-1 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Myogenic differentiation |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Rb |
| 書誌情報 |
en : BMC Cell Biology
巻 16,
p. 8,
発行日 2015-03-11
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| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
26618850 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA12034785 |
| 出版者 |
|
|
出版者 |
Springer Nature |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
BioMed Central |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
© 2015 Nagano et al.; licensee BioMed Central. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
| EID |
|
|
識別子 |
295410 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
bmccb_16_8.pdf (1.15 MB)
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