| Item type |
文献 / Documents(1) |
| 公開日 |
2021-01-15 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.1038/s41467-018-07481-7 |
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関連名称 |
10.1038/s41467-018-07481-7 |
| 出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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タイトル |
Force-dependent allostery of the α-catenin actinbinding domain controls adherens junction dynamics and functions |
| 著者 |
イシヤマ, ノボル
Sarpal, Ritu
Wood, Megan N.
Barrick, Samantha K.
ニシカワ, タダテル
ハヤシ, ハナコ
Kobb, Anna B.
Flozak, Annette S.
Yemelyanov, Alex
Fernandez-Gonzalez, Rodrigo
米村, 重信
Leckband, Deborah E.
Gottardi, Cara J.
Tepass, Ulrich
イクラ, ミツヒコ
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| 抄録 |
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内容記述 |
α-catenin is a key mechanosensor that forms force-dependent interactions with F-actin, thereby coupling the cadherin-catenin complex to the actin cytoskeleton at adherens junctions (AJs). However, the molecular mechanisms by which α-catenin engages F-actin under tension remained elusive. Here we show that the α1-helix of the α-catenin actin-binding domain (αcat-ABD) is a mechanosensing motif that regulates tension-dependent F-actin binding and bundling. αcat-ABD containing an α1-helix-unfolding mutation (H1) shows enhanced binding to F-actin in vitro. Although full-length α-catenin-H1 can generate epithelial monolayers that resist mechanical disruption, it fails to support normal AJ regulation in vivo. Structural and simulation analyses suggest that α1-helix allosterically controls the actin-binding residue V796 dynamics. Crystal structures of αcat-ABD-H1 homodimer suggest that α-catenin can facilitate actin bundling while it remains bound to E-cadherin. We propose that force-dependent allosteric regulation of αcat-ABD promotes dynamic interactions with F-actin involved in actin bundling, cadherin clustering, and AJ remodeling during tissue morphogenesis. |
| 書誌情報 |
en : Nature Communications
巻 9,
p. 5121,
発行日 2018-11-30
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
20411723 |
| 収録物ID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA12645905 |
| 出版者 |
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出版者 |
Springer Nature |
| 権利情報 |
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権利情報 |
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
© The Author(s) 2018 |
| EID |
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識別子 |
355307 |
| 言語 |
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言語 |
eng |
ncomms_9_5121.pdf (8.57 MB)
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