| アイテムタイプ |
文献 / Documents(1) |
| 公開日 |
2020-07-17 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.1371/journal.pone.0229948 |
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関連名称 |
10.1371/journal.pone.0229948 |
| 出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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タイトル |
Nanosecond pulsed electric fields induce the integrated stress response via reactive oxygen species-mediated heme-regulated inhibitor (HRI) activation |
| タイトル別表記 |
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その他のタイトル |
nsPEFs induce the ISR via ROS-mediated HRI activation |
| 著者 |
濱田, 良真
フルモト, ユウジ
イズタニ, アキラ
タニウチ, シュウスケ
三宅, 雅人
親泊, 美帆
WEKO
478
e-Rad_Researcher
00596158
| ja |
親泊, 美帆
親泊, 美帆
ISNI
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| ja-Kana |
オヤドマリ, ミホ
オヤドマリ, ミホ
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| en |
Oyadomari, Miho
Oyadomari, Miho
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Search repository
寺西, 研二
下村, 直行
親泊, 政一
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| 抄録 |
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内容記述 |
The integrated stress response (ISR) is one of the most important cytoprotective mechanisms and is integrated by phosphorylation of the α subunit of eukaryotic translation initiation factor 2 (eIF2α). Four eIF2α kinases, heme-regulated inhibitor (HRI), double-stranded RNA-dependent protein kinase (PKR), PKR-like endoplasmic reticulum kinase (PERK), and general control nonderepressible 2 (GCN2), are activated in response to several stress conditions. We previously reported that nanosecond pulsed electric fields (nsPEFs) are a potential therapeutic tool for ISR activation. In this study, we examined which eIF2α kinase is activated by nsPEF treatment. To assess the responsible eIF2α kinase, we used previously established eIF2α kinase quadruple knockout (4KO) and single eIF2α kinase-rescued 4KO mouse embryonic fibroblast (MEF) cells. nsPEFs 70 ns in duration with 30 kV/cm electric fields caused eIF2α phosphorylation in wild-type (WT) MEF cells. On the other hand, nsPEF-induced eIF2α phosphorylation was completely abolished in 4KO MEF cells and was recovered by HRI overexpression. CM-H2DCFDA staining showed that nsPEFs generated reactive oxygen species (ROS), which activated HRI. nsPEF-induced eIF2α phosphorylation was blocked by treatment with the ROS scavenger N-acetyl-L-cysteine (NAC). Our results indicate that the eIF2α kinase HRI is responsible for nsPEF-induced ISR activation and is activated by nsPEF-generated ROS. |
| 書誌情報 |
en : PLOS ONE
巻 15,
号 3,
p. e0229948,
発行日 2020-03-10
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
19326203 |
| 出版者 |
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出版者 |
PLOS |
| 権利情報 |
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権利情報 |
© 2020 Hamada et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| EID |
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識別子 |
363648 |
| 言語 |
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言語 |
eng |
pone_15_3_e0229948.pdf (1.66 MB)
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