| Item type |
文献 / Documents(1) |
| 公開日 |
2020-07-29 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1371/journal.ppat.1004505 |
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言語 |
ja |
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関連名称 |
10.1371/journal.ppat.1004505 |
| 出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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タイトル |
GAPDH-A Recruits a Plant Virus Movement Protein to Cortical Virus Replication Complexes to Facilitate Viral Cell-to-Cell Movement |
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言語 |
en |
| タイトル別表記 |
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その他のタイトル |
GAPDH-A Facilitates Intercellular Movement of RCNMV |
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言語 |
en |
| 著者 |
カイドウ, マサノリ
アベ, カズトモ
ミネ, アキラ
ヒョウドウ, キワム
谷口, 貴子
谷口, 寿章
ミセ, カズユキ
オクノ, テツロウ
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
The formation of virus movement protein (MP)-containing punctate structures on the cortical endoplasmic reticulum is required for efficient intercellular movement of Red clover necrotic mosaic virus (RCNMV), a bipartite positive-strand RNA plant virus. We found that these cortical punctate structures constitute a viral replication complex (VRC) in addition to the previously reported aggregate structures that formed adjacent to the nucleus. We identified host proteins that interacted with RCNMV MP in virus-infected Nicotiana benthamiana leaves using a tandem affinity purification method followed by mass spectrometry. One of these host proteins was glyceraldehyde 3-phosphate dehydrogenase-A (NbGAPDH-A), which is a component of the Calvin-Benson cycle in chloroplasts. Virus-induced gene silencing of NbGAPDH-A reduced RCNMV multiplication in the inoculated leaves, but not in the single cells, thereby suggesting that GAPDH-A plays a positive role in cell-to-cell movement of RCNMV. The fusion protein of NbGAPDH-A and green fluorescent protein localized exclusively to the chloroplasts. In the presence of RCNMV RNA1, however, the protein localized to the cortical VRC as well as the chloroplasts. Bimolecular fluorescence complementation assay and GST pulldown assay confirmed in vivo and in vitro interactions, respectively, between the MP and NbGAPDH-A. Furthermore, gene silencing of NbGAPDH-A inhibited MP localization to the cortical VRC. We discuss the possible roles of NbGAPDH-A in the RCNMV movement process. |
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言語 |
en |
| 書誌情報 |
en : PLOS Pathogens
巻 10,
号 11,
p. e1004505,
発行日 2014-11-20
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
15537366 |
| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
15537374 |
| 出版者 |
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出版者 |
PLOS |
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言語 |
en |
| 権利情報 |
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言語 |
en |
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権利情報 |
© 2014 Kaido et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| EID |
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識別子 |
290285 |
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識別子タイプ |
URI |
| 言語 |
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言語 |
eng |
plospathog_10_11_e1004505.pdf (2.86 MB)
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