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A change in the zinc ion concentration reflects the maturation of insulin-producing cells generated from adipose-derived mesenchymal stem cells
https://tokushima-u.repo.nii.ac.jp/records/2007872
https://tokushima-u.repo.nii.ac.jp/records/20078723036be32-04cb-4321-a2b6-54430096bb6a
| 名前 / ファイル | ライセンス | アクション |
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k3428_abstract_review.pdf (231 KB)
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k3428_fulltext.pdf (2.99 MB)
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| Item type | 文献 / Documents(1) | |||||||||||
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| 公開日 | 2020-07-07 | |||||||||||
| アクセス権 | ||||||||||||
| アクセス権 | open access | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||||
| 資源タイプ | doctoral thesis | |||||||||||
| 出版社版DOI | ||||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | https://doi.org/10.1038/s41598-019-55172-0 | |||||||||||
| 言語 | ja | |||||||||||
| 関連名称 | 10.1038/s41598-019-55172-0 | |||||||||||
| 出版タイプ | ||||||||||||
| 出版タイプ | NA | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_be7fb7dd8ff6fe43 | |||||||||||
| タイトル | ||||||||||||
| タイトル | A change in the zinc ion concentration reflects the maturation of insulin-producing cells generated from adipose-derived mesenchymal stem cells | |||||||||||
| 言語 | en | |||||||||||
| タイトル別表記 | ||||||||||||
| その他のタイトル | 亜鉛イオン濃度変化は脂肪由来幹細胞から作成するインスリン産生細胞の成熟を反映する | |||||||||||
| 言語 | ja | |||||||||||
| 著者 |
太田, 昇吾
× 太田, 昇吾
× 池本, 哲也× 和田, 佑馬× 齋藤, 裕× 山田, 眞一郎× 居村, 暁× 森根, 裕二× 島田, 光生 |
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| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | The generation of insulin-producing cells (IPCs) from pluripotent stem cells could be a breakthrough treatment for type 1 diabetes. However, development of new techniques is needed to exclude immature cells for clinical application. Dithizone staining is used to evaluate IPCs by detecting zinc. We hypothesised that zinc ion (Zn2+) dynamics reflect the IPC maturation status. Human adipose-derived stem cells were differentiated into IPCs by our two-step protocol using two-dimensional (2D) or 3D culture. The stimulation indexes of 2D -and 3D-cultured IPCs on day 21 were 1.21 and 3.64 (P < 0.05), respectively. The 3D-cultured IPCs were stained with dithizone during culture, and its intensity calculated by ImageJ reached the peak on day 17 (P < 0.05). Blood glucose levels of streptozotocin-induced diabetic nude mice were normalised (4/4,100%) after transplantation of 96 3D-cultured IPCs. Zn2+ concentration changes in the medium of 3D cultures had a negative value in the early period and a large positive value in the latter period. This study suggests that Zn2+ dynamics based on our observations and staining of zinc transporters have critical roles in the differentiation of IPCs, and that their measurement might be useful to evaluate IPC maturation as a non-destructive method. | |||||||||||
| 言語 | en | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Insulin producing cell (IPC) | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | adipose derived stem cell (ADSC) | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | zinc ion (Zn) | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | maturation | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | differentiation | |||||||||||
| 書誌情報 |
en : Scientific Reports 巻 9, p. 18731, 発行日 2019-12-10 |
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| 収録物ID | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 20452322 | |||||||||||
| 出版者 | ||||||||||||
| 出版者 | Springer Nature | |||||||||||
| 言語 | en | |||||||||||
| 備考 | ||||||||||||
| 言語 | ja | |||||||||||
| 値 | 内容要旨・審査要旨・論文本文の公開 本論文は, 著者Shogo Ohtaの学位論文として提出され, 学位審査・授与の対象となっている。 学位授与者所属 : 徳島大学大学院医科学教育部（医学専攻） |
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| 権利情報 | ||||||||||||
| 言語 | en | |||||||||||
| 権利情報 | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | |||||||||||
| EID | ||||||||||||
| 識別子 | 367223 | |||||||||||
| 識別子タイプ | URI | |||||||||||
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| 言語 | eng | |||||||||||
| 報告番号 | ||||||||||||
| 学位授与番号 | 甲第3428号 | |||||||||||
| 学位記番号 | ||||||||||||
| 言語 | ja | |||||||||||
| 値 | 甲医第1458号 | |||||||||||
| 学位授与年月日 | ||||||||||||
| 学位授与年月日 | 2020-06-25 | |||||||||||
| 学位名 | ||||||||||||
| 言語 | ja | |||||||||||
| 学位名 | 博士（医学） | |||||||||||
| 学位授与機関 | ||||||||||||
| 言語 | ja | |||||||||||
| 学位授与機関名 | 徳島大学 | |||||||||||
