Item type |
文献 / Documents(1) |
公開日 |
2020-07-30 |
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アクセス権 |
open access |
資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
出版社版DOI |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1038/s41598-019-47257-7 |
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言語 |
ja |
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関連名称 |
10.1038/s41598-019-47257-7 |
出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
タイトル |
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タイトル |
In vitro and in vivo effects of insulin-producing cells generated by xeno-antigen free 3D culture with RCP piece |
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言語 |
en |
著者 |
池本, 哲也
Feng, Rui
岩橋, 衆一
山田, 眞一郎
齋藤, 裕
森根, 裕二
居村, 暁
松久, 宗英
島田, 光生
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抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
To establish widespread cell therapy for type 1 diabetes mellitus, we aimed to develop an effective protocol for generating insulin-producing cells (IPCs) from adipose-derived stem cells (ADSCs). We established a 3D culture using a human recombinant peptide (RCP) petaloid μ-piece with xeno-antigen free reagents. Briefly, we employed our two-step protocol to differentiate ADSCs in 96-well dishes and cultured cells in xeno-antigen free reagents with 0.1 mg/mL RCP μ-piece for 7 days (step 1), followed by addition of histone deacetylase inhibitor for 14 days (step 2). Generated IPCs were strongly stained with dithizone, anti-insulin antibody at day 21, and microstructures resembling insulin secretory granules were detected by electron microscopy. Glucose stimulation index (maximum value, 4.9) and MAFA mRNA expression were significantly higher in 3D cultured cells compared with conventionally cultured cells (P < 0.01 and P < 0.05, respectively). The hyperglycaemic state of streptozotocin-induced diabetic nude mice converted to normoglycaemic state around 14 days after transplantation of 96 IPCs under kidney capsule or intra-mesentery. Histological evaluation revealed that insulin and C-peptide positive structures existed at day 120. Our established xeno-antigen free and RCP petaloid μ-piece 3D culture method for generating IPCs may be suitable for clinical application, due to the proven effectiveness in vitro and in vivo. |
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言語 |
en |
書誌情報 |
en : Scientific Reports
巻 9,
p. 10759,
発行日 2019-07-24
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収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
20452322 |
出版者 |
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出版者 |
Springer Nature |
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言語 |
en |
権利情報 |
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言語 |
en |
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権利情報 |
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
EID |
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識別子 |
355298 |
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識別子タイプ |
URI |
言語 |
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言語 |
eng |