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Low Electric Treatment activates Rho GTPase via Heat Shock Protein 90 and Protein Kinase C for Intracellular Delivery of siRNA
https://tokushima-u.repo.nii.ac.jp/records/2007877
https://tokushima-u.repo.nii.ac.jp/records/2007877cac54402-92d2-4143-a1a2-351840a07d1f
| 名前 / ファイル | ライセンス | アクション |
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srep_9_4114.pdf (2.06 MB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||||
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| 公開日 | 2020-07-31 | |||||||||||||||||||
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| アクセス権 | open access | |||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||
| 出版社版DOI | ||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1038/s41598-019-40904-z | |||||||||||||||||||
| 関連名称 | 10.1038/s41598-019-40904-z | |||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||
| タイトル | ||||||||||||||||||||
| タイトル | Low Electric Treatment activates Rho GTPase via Heat Shock Protein 90 and Protein Kinase C for Intracellular Delivery of siRNA | |||||||||||||||||||
| 著者 |
Hasan, Mahadi
× Hasan, Mahadi
× ハマ, ススム
× 小暮, 健太朗
WEKO
1139
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| 内容記述 | Low electric treatment (LET) promotes intracellular delivery of naked siRNA by altering cellular physiology. However, which signaling molecules and cellular events contribute to LET-mediated siRNA uptake are unclear. Here, we used isobaric tags in relative and absolute quantification (iTRAQ) proteomic analysis to identify changes in the levels of phosphorylated proteins that occur during cellular uptake of siRNA promoted by LET. iTRAQ analysis revealed that heat shock protein 90 (Hsp90)α and myristoylated alanine-rich C-kinase substrate (Marcks) were highly phosphorylated following LET of NIH 3T3 cells, but not untreated cells. Furthermore, the levels of phosphorylated Hsp90α and protein kinase C (PKC)γ were increased by LET both with siRNA and liposomes having various physicochemical properties used as model macromolecules, suggesting that PKCγ activated partly by Ca2+ influx as well as Hsp90 chaperone function were involved in LET-mediated cellular siRNA uptake. Furthermore, LET with siRNA induced activation of Rho GTPase via Hsp90 and PKC, which could contribute to cellular siRNA uptake accompanied by actin cytoskeleton remodeling. Collectively, our results suggested that LET-induced Rho GTPase activation via Hsp90 and PKC would participate in actin-dependent cellular uptake of siRNA. | |||||||||||||||||||
| 書誌情報 |
en : Scientific Reports 巻 9, p. 4114, 発行日 2019-03-11 |
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| 収録物識別子タイプ | ISSN | |||||||||||||||||||
| 収録物識別子 | 20452322 | |||||||||||||||||||
| 出版者 | ||||||||||||||||||||
| 出版者 | Springer Nature | |||||||||||||||||||
| 権利情報 | ||||||||||||||||||||
| 権利情報 | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | |||||||||||||||||||
| EID | ||||||||||||||||||||
| 識別子 | 359919 | |||||||||||||||||||
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| 言語 | eng | |||||||||||||||||||
