| Item type |
文献 / Documents(1) |
| 公開日 |
2020-08-04 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1038/s41598-019-53259-2 |
|
|
言語 |
ja |
|
|
関連名称 |
10.1038/s41598-019-53259-2 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Identification of candidate molecular targets of the novel antineoplastic antimitotic NP-10 |
|
言語 |
en |
| 著者 |
ヨコヤマ, タクヤ
ユクヒロ, マサキ
イワサキ
タナカ, チカ
サンコダ, カズナリ
フジワラ, リサ
シブタ, アツシ
ヒガシ, タイシ
モトヤマ, ケイイチ
アリマ, ヒデトシ
ヨシダ, カズマサ
スギモト, ノゾミ
モリモト, ヒロユキ
小迫, 英尊
オオシマ, タカシ
フジタ, マサトシ
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
We previously reported the identification of a novel antimitotic agent with carbazole and benzohydrazide structures: N′-[(9-ethyl-9H-carbazol-3-yl)methylene]-2-iodobenzohydrazide (code number NP-10). However, the mechanism(s) underlying the cancer cell-selective inhibition of mitotic progression by NP-10 remains unclear. Here, we identified NP-10-interacting proteins by affinity purification from HeLa cell lysates using NP-10-immobilized beads followed by mass spectrometry. The results showed that several mitosis-associated factors specifically bind to active NP-10, but not to an inactive NP-10 derivative. Among them, NUP155 and importin β may be involved in NP-10-mediated mitotic arrest. Because NP-10 did not show antitumor activity in vivo in a previous study, we synthesized 19 NP-10 derivatives to identify more effective NP-10-related compounds. HMI83-2, an NP-10-related compound with a Cl moiety, inhibited HCT116 cell tumor formation in nude mice without significant loss of body weight, suggesting that HMI83-2 is a promising lead compound for the development of novel antimitotic agents. |
|
言語 |
en |
| 書誌情報 |
en : Scientific Reports
巻 9,
p. 16825,
発行日 2019-11-14
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
20452322 |
| 出版者 |
|
|
出版者 |
Springer Nature |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| EID |
|
|
識別子 |
360970 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
srep_9_16825.pdf (4.27 MB)
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