| Item type |
文献 / Documents(1) |
| 公開日 |
2020-08-04 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1038/s41598-019-48323-w |
|
|
言語 |
ja |
|
|
関連名称 |
10.1038/s41598-019-48323-w |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
The Fab portion of immunoglobulin G contributes to its binding to Fcγ receptor III |
|
言語 |
en |
| 著者 |
ヨゴ, リナ
ヤマグチ, ユウキ
ワタナベ, ヒロキ
ヤギ, ヒロカズ
サトウ, タダシ
ナカニシ, マヒト
鬼塚, 正義
大政, 健史
シマダ, マリ
マルノ, タカヒロ
トリス, テツオ
ワタナベ, シオ
ヒゴ, ダイスケ
ウチハシ, タカユキ
ヤナカ, サエコ
ウチヤマ, ススム
カトウ, コウイチ
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Most cells active in the immune system express receptors for antibodies which mediate a variety of defensive mechanisms. These receptors interact with the Fc portion of the antibody and are therefore collectively called Fc receptors. Here, using high-speed atomic force microscopy, we observe interactions of human, humanized, and mouse/human-chimeric immunoglobulin G1 (IgG1) antibodies and their cognate Fc receptor, FcγRIIIa. Our results demonstrate that not only Fc but also Fab positively contributes to the interaction with the receptor. Furthermore, hydrogen/deuterium exchange mass spectrometric analysis reveals that the Fab portion of IgG1 is directly involved in its interaction with FcγRIIIa, in addition to the canonical Fc-mediated interaction. By targeting the previously unidentified receptor-interaction sites in IgG-Fab, our findings could inspire therapeutic antibody engineering. |
|
言語 |
en |
| 書誌情報 |
en : Scientific Reports
巻 9,
p. 11957,
発行日 2019-08-16
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
20452322 |
| 出版者 |
|
|
出版者 |
Springer Nature |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| EID |
|
|
識別子 |
356929 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
srep_9_11957.pdf (1.59 MB)
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