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Peptide aptamer-modified single-walled carbon nanotube-based transistors for high-performance biosensors
https://tokushima-u.repo.nii.ac.jp/records/2007894
https://tokushima-u.repo.nii.ac.jp/records/200789470b34926-d422-4184-b731-9b90aecce28d
| 名前 / ファイル | ライセンス | アクション |
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srep_7_17881.pdf (3 MB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||||||||||||||||||||||||||||
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| 公開日 | 2020-08-06 | |||||||||||||||||||||||||||||||||||||||||||
| アクセス権 | ||||||||||||||||||||||||||||||||||||||||||||
| アクセス権 | open access | |||||||||||||||||||||||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||||||||||||||||||||||||
| 出版社版DOI | ||||||||||||||||||||||||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1038/s41598-017-18169-1 | |||||||||||||||||||||||||||||||||||||||||||
| 関連名称 | 10.1038/s41598-017-18169-1 | |||||||||||||||||||||||||||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||||||||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||||||||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||||||||||||||||||||||||
| タイトル | ||||||||||||||||||||||||||||||||||||||||||||
| タイトル | Peptide aptamer-modified single-walled carbon nanotube-based transistors for high-performance biosensors | |||||||||||||||||||||||||||||||||||||||||||
| 著者 |
Tung, Nguyen Thanh
× Tung, Nguyen Thanh
× Tue, Phan Trong
× Lien, Truong Thi Ngoc
× 大野, 恭秀
WEKO
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× マエハシ, ケンゾウ
× マツモト, カズヒコ
× ニシガキ, コウイチ
× Biyani, Manish
× タカムラ, ユズル
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| 抄録 | ||||||||||||||||||||||||||||||||||||||||||||
| 内容記述 | Biosensors employing single-walled carbon nanotube field-effect transistors (SWCNT FETs) offer ultimate sensitivity. However, besides the sensitivity, a high selectivity is critically important to distinguish the true signal from interference signals in a non-controlled environment. This work presents the first demonstration of the successful integration of a novel peptide aptamer with a liquid-gated SWCNT FET to achieve highly sensitive and specific detection of Cathepsin E (CatE), a useful prognostic biomarker for cancer diagnosis. Novel peptide aptamers that specifically recognize CatE are engineered by systemic in vitro evolution. The SWCNTs were firstly grown using the thermal chemical vapor deposition (CVD) method and then were employed as a channel to fabricate a SWCNT FET device. Next, the SWCNTs were functionalized by noncovalent immobilization of the peptide aptamer using 1-pyrenebutanoic acid succinimidyl ester (PBASE) linker. The resulting FET sensors exhibited a high selectivity (no response to bovine serum albumin and cathepsin K) and label-free detection of CatE at unprecedentedly low concentrations in both phosphate-buffered saline (2.3 pM) and human serum (0.23 nM). Our results highlight the use of peptide aptamer-modified SWCNT FET sensors as a promising platform for near-patient testing and point-of-care testing applications. | |||||||||||||||||||||||||||||||||||||||||||
| 書誌情報 |
en : Scientific Reports 巻 7, p. 17881, 発行日 2017-12-20 |
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| 収録物識別子タイプ | ISSN | |||||||||||||||||||||||||||||||||||||||||||
| 収録物識別子 | 20452322 | |||||||||||||||||||||||||||||||||||||||||||
| 出版者 | ||||||||||||||||||||||||||||||||||||||||||||
| 出版者 | Springer Nature | |||||||||||||||||||||||||||||||||||||||||||
| 権利情報 | ||||||||||||||||||||||||||||||||||||||||||||
| 権利情報 | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | |||||||||||||||||||||||||||||||||||||||||||
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| 識別子 | 353985 | |||||||||||||||||||||||||||||||||||||||||||
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| 言語 | eng | |||||||||||||||||||||||||||||||||||||||||||
