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Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing
https://tokushima-u.repo.nii.ac.jp/records/2007904
https://tokushima-u.repo.nii.ac.jp/records/2007904c847adf1-ba2e-4297-8de4-91c75af28c10
| 名前 / ファイル | ライセンス | アクション |
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srep_5_11315.pdf (2.8 MB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||
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| 公開日 | 2020-08-06 | |||||||||||||||||
| アクセス権 | ||||||||||||||||||
| アクセス権 | open access | |||||||||||||||||
| 資源タイプ | ||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||
| 出版社版DOI | ||||||||||||||||||
| 関連識別子 | https://doi.org/10.1038/srep11315 | |||||||||||||||||
| 関連名称 | 10.1038/srep11315 | |||||||||||||||||
| 出版タイプ | ||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||
| タイトル | ||||||||||||||||||
| タイトル | Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing | |||||||||||||||||
| 著者 |
ハシモト, マサカズ
× ハシモト, マサカズ
× 竹本, 龍也
WEKO
680
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| 抄録 | ||||||||||||||||||
| 内容記述 | Recent use of the CRISPR/Cas9 system has dramatically reduced the time required to produce mutant mice, but the involvement of a time-consuming microinjection step still hampers its application for high-throughput genetic analysis. Here we developed a simple, highly efficient and large-scale genome editing method, in which the RNAs for the CRISPR/Cas9 system are electroporated into zygotes rather than microinjected. We used this method to perform single-stranded oligodeoxynucleotide (ssODN)-mediated knock-in in mouse embryos. This method facilitates large-scale genetic analysis in the mouse. | |||||||||||||||||
| 書誌情報 |
en : Scientific Reports 巻 5, p. 11315, 発行日 2015-06-11 |
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| 収録物ID | ||||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||||
| 収録物識別子 | 20452322 | |||||||||||||||||
| 出版者 | ||||||||||||||||||
| 出版者 | Springer Nature | |||||||||||||||||
| 権利情報 | ||||||||||||||||||
| 権利情報 | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ | |||||||||||||||||
| EID | ||||||||||||||||||
| 識別子 | 311988 | |||||||||||||||||
| 言語 | ||||||||||||||||||
| 言語 | eng | |||||||||||||||||
