| Item type |
文献 / Documents(1) |
| 公開日 |
2020-08-07 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
https://doi.org/10.1136/vetreco-2015-000124 |
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|
言語 |
ja |
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|
関連名称 |
10.1136/vetreco-2015-000124 |
| 出版タイプ |
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|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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|
タイトル |
Genotyping and characterisation of the secretory lipolytic enzymes of Malassezia pachydermatis isolates collected from dogs |
|
言語 |
en |
| 著者 |
テラモト, ヒデシ
クメダ, ユウコ
横井川, 久己男
ホソミ, コウジ
コザキ, シュンジ
ムカモト, マサフミ
コウダ, トモコ
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Introduction: Malassezia species are commensals of normal skin microbial flora of humans and animals. These may become pathogenic under certain conditions such as those associated with atopic dermatitis or otitis externa in dogs.
Material and methods: Isolates of Malassezia pachydermatis were obtained from 27 dogs with healthy external ears and 32 dogs with otitis externa. Isolates were characterised on the basis of their first internal transcribed spacer (ITS) and internal spacer 1 (IGS1) sequences. Their extracellular lipase and phospholipase activity were also analysed. Three types of phospholipase inhibitor were used to identify the subclasses of phospholipase associated with otitis externa.
Results: The clinical isolates were classified into three ITS and three IGS1 sequence types. No significant differences in pathogenicity were detected among the ITS or IGS1 genotypes, and all of the isolates exhibited similar levels of lipase activity. The isolates derived from the dogs with otitis externa showed significantly higher phospholipase activity than those obtained from the dogs with healthy external ears. A phospholipase D inhibitor reduced the phospholipase activity of the isolates obtained from the dogs with otitis externa.
Conclusions: This study did not show any significant differences in pathogenicity among the ITS or IGS1 genotypes but does suggest that phospholipase D might be one of the virulence factors involved in the inflammation of the external ear caused by M. pachydermatis. |
|
言語 |
en |
| 書誌情報 |
en : Veterinary Record Open
巻 2,
号 2,
p. e000124,
発行日 2015-08-14
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
20526113 |
| 出版者 |
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|
出版者 |
British Veterinary Association |
|
言語 |
en |
| 出版者 |
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|
出版者 |
BMJ Publishing Group |
|
言語 |
en |
| 権利情報 |
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|
言語 |
en |
|
権利情報 |
This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
| EID |
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|
識別子 |
310594 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
vetreco_2_2_e000124.pdf (894 KB)
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