| Item type |
文献 / Documents(1) |
| 公開日 |
2020-08-19 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.1267/ahc.11041 |
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関連名称 |
10.1267/ahc.11041 |
| 出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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タイトル |
Interaction of Protein Phosphatase 1δ with Nucleophosmin in Human Osteoblastic Cells |
| タイトル別表記 |
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その他のタイトル |
Protein Phosphatase 1δ with Nucleophosmin |
| 著者 |
羽地, 達次
寺町, 順平
ヒラシマ, カンジ
キムラ, コウジ
森本, 景之
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| 抄録 |
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内容記述 |
Protein phosphorylation and dephosphorylation has been recognized as an essential mechanism in the regulation of cellular metabolism and function in various tissues. Serine and threonine protein phosphatases (PP) are divided into four categories: PP1, PP2A, PP2B, and PP2C. At least four isoforms of PP1 catalytic subunit in rat, PP1α, PP1γ1, PP1γ2, and PP1δ, were isolated. In the present study, we examined the localization and expression of PP1δ in human osteoblastic Saos-2 cells. Anti-PP1δ antibody recognized a protein present in the nucleolar regions in Saos-2 cells. Cellular fractionation revealed that PP1δ is a 37 kDa protein localized in the nucleolus. Nucleophosmin is a nucleolar phosphoprotein and located mainly in the nucleolus. Staining pattern of nucleophosmin in Saos-2 cells was similar to that of PP1δ. PP1δ and nucleophosmin were specifically stained as dots in the nucleus. Dual fluorescence images revealed that PP1δ and nucleophosmin were localized in the same regions in the nucleolus. Similar distribution patterns of PP1δ and nucleophosmin were observed in osteoblastic MG63 cells. The interaction of PP1δ and nucleophosmin was also shown by immunoprecipitation and Western analysis. These results indicated that PP1δ associate with nucleophosmin directly in the nucleolus and suggested that nucleophosmin is one of the candidate substrate for PP1δ. |
| キーワード |
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主題 |
protein phosphatase |
| キーワード |
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主題 |
nucleophosmin |
| キーワード |
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主題 |
osteoblast |
| 書誌情報 |
en : Acta Histochemica et Cytochemica
巻 45,
号 1,
p. 1-7,
発行日 2011-11-05
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
00445991 |
| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
13475800 |
| 収録物ID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA00508022 |
| 出版者 |
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出版者 |
The Japan Society of Histochemistry and Cytochemistry |
| 権利情報 |
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|
権利情報 |
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| EID |
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|
識別子 |
251594 |
| 言語 |
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|
言語 |
eng |
ahc_45_1_1.pdf (1.32 MB)
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