| Item type |
文献 / Documents(1) |
| 公開日 |
2020-09-16 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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|
関連識別子 |
https://doi.org/10.1016/j.bbrep.2015.05.006 |
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関連名称 |
10.1016/j.bbrep.2015.05.006 |
| 出版タイプ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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タイトル |
Interleukin-6/STAT pathway is responsible for the induction of gene expression of REG Iα, a new auto-antigen in Sjögren׳s syndrome patients, in salivary duct epithelial cells |
| 著者 |
フジムラ, タカノリ
フジモト, タカシ
イタヤ ヒロナカ, アサコ
ミヤオカ, トモコ
ヨシモト, キヨミ
ヤマウチ, アキヨ
サクラモト ツチダ, スミヨ
| ja |
サクラモト ツチダ, スミヨ
|
| ja-Kana |
サクラモト ツチダ, スミヨ
|
| en |
Sakuramoto-Tsuchida, Sumiyo
|
Search repository
コンドウ, サオリ
タケダ, マイコ
ツジナカ, ヒロキ
東, 雅之
タナカ, ヤスヒト
タカサワ, シン
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| 抄録 |
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内容記述 |
The regenerating gene, Reg, was originally isolated from a rat regenerating islet cDNA library, and its human homolog was named REG Iα. Recently, we reported that REG Iα mRNA as well as its product were overexpressed in ductal epithelial cells in the minor salivary glands of Sjögren׳s syndrome (SS) patients. This study was undertaken to elucidate the role of cytokines and the subsequent intracellular mechanism for induction of REG Iα in the salivary glands of SS patients. We prepared a reporter plasmid containing REG Iα promoter (−1190/+26) upstream of a luciferase reporter gene. The promoter plasmid was introduced by lipofection into human NS-SV-DC and rat A5 salivary ductal cells. The cells were treated with interleukin (IL)-6, IL-8, and a combination of the two. Thereafter transcriptional activity of REG Iα was measured by luciferase assay. We found that IL-6 stimulation, but not IL-8, significantly enhanced the REG Iα promoter activity in salivary ductal cells. Deletion analysis revealed that the region of −141 to −117 of the REG Iα gene was responsible for the promoter activation by IL-6, which contains a consensus sequence for signal transduction and activation of transcription (STAT). The introduction of siRNA for human STAT3 abolished IL-6-induced REG Iα transcription. These results showed that IL-6 stimulation induced REG Iα transcription through STAT3 activation and binding to the consensus sequence of REG Iα promoter in salivary ductal cells. This IL-6/STAT dependent REG Iα induction might play a role in the pathogenesis of SS. |
| キーワード |
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主題 |
Regenerating gene |
| キーワード |
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主題 |
Interleukin-6 |
| キーワード |
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|
主題 |
Sjögren׳s syndrome |
| キーワード |
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|
主題 |
Janus kinase |
| キーワード |
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|
主題 |
Signal transducer and activator of transcription |
| 書誌情報 |
en : Biochemistry and Biophysics Reports
巻 2,
p. 69-74,
発行日 2015-05-30
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
24055808 |
| 出版者 |
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出版者 |
Elsevier |
| 権利情報 |
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|
権利情報 |
© 2015 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| EID |
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|
識別子 |
297434 |
| 言語 |
|
|
言語 |
eng |
bbrep_2_69.pdf (530 KB)
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