| Item type |
文献 / Documents(1) |
| 公開日 |
2020-08-17 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
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|
関連識別子 |
https://doi.org/10.1038/s42004-019-0250-0 |
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|
関連名称 |
10.1038/s42004-019-0250-0 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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|
タイトル |
Development of a 1,3a,6a-triazapentalene derivative as a compact and thiol-specific fluorescent labeling reagent |
| 著者 |
中山, 淳
オオタニ, アキラ
猪熊, 翼
辻, 大輔
ムカイヤマ, ハルカ
ナカヤマ, アキラ
伊藤, 孝司
大髙, 章
タニノ, ケイジ
難波, 康祐
|
| 抄録 |
|
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内容記述 |
For the fluorescence imaging of biologically active small compounds, the development of compact fluorophores that do not perturb bioactivity is required. Here we report a compact derivative of fluorescent 1,3a,6a-triazapentalenes, 2-isobutenylcarbonyl-1,3a,6a-triazapentalene (TAP-VK1), as a fluorescent labeling reagent. The reaction of TAP-VK1 with various aliphatic thiols proceeds smoothly to afford the corresponding 1,4-adducts in high yields, and nucleophiles other than thiols do not react. After the addition of thiol groups in dichloromethane, the emission maximum of TAP-VK1 shifts to a shorter wavelength and the fluorescence intensity is substantially increased. The utility of TAP-VK1 as a compact fluorescent labeling reagent is clearly demonstrated by the labeling of Captopril, which is a small molecular drug for hypertension. The successful imaging of Captopril, one of the most compact drugs, in this study demonstrates the usefulness of compact fluorophores for mechanistic studies. |
| 書誌情報 |
en : Communications Chemistry
巻 3,
p. 6,
発行日 2020-01-09
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
23993669 |
| 出版者 |
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出版者 |
Springer Nature |
| 権利情報 |
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|
権利情報 |
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| EID |
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|
識別子 |
362825 |
| 言語 |
|
|
言語 |
eng |
commschem_3_6.pdf (1.87 MB)
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