| Item type |
文献 / Documents(1) |
| 公開日 |
2020-08-21 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.3390/ijms21176005 |
|
|
言語 |
ja |
|
|
関連名称 |
10.3390/ijms21176005 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Role of CGRP in Neuroimmune Interaction via NF-κB Signaling Genes in Glial Cells of Trigeminal Ganglia |
|
言語 |
en |
| 著者 |
Afroz, Shaista
新垣, 理恵子
岩浅, 匠真
Waskitho, Arief
大島, 正充
松香, 芳三
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Activation of the trigeminal system causes the release of various neuropeptides, cytokines, and other immune mediators. Calcitonin gene-related peptide (CGRP), which is a potent algogenic mediator, is expressed in the peripheral sensory neurons of trigeminal ganglion (TG). It affects the inflammatory responses and pain sensitivity by modulating the activity of glial cells. The primary aim of this study was to use array analysis to investigate the effect of CGRP on the glial cells of TG in regulating nuclear factor kappa B (NF-κB) signaling genes and to further check if CGRP in the TG can affect neuron-glia activation in the spinal trigeminal nucleus caudalis. The glial cells of TG were stimulated with CGRP or Minocycline (Min) + CGRP. The effect on various genes involved in NF-κB signaling pathway was analyzed compared to no treatment control condition using a PCR array analysis. CGRP, Min + CGRP or saline was directly injected inside the TG and the effect on gene expression of Egr1, Myd88 and Akt1 and protein expression of cleaved Caspase3 (cleav Casp3) in the TG, and c-Fos and glial fibrillary acidic protein (GFAP) in the spinal section containing trigeminal nucleus caudalis was analyzed. Results showed that CGRP stimulation resulted in the modulation of several genes involved in the interleukin 1 signaling pathway and some genes of the tumor necrosis factor pathway. Minocycline pre-treatment resulted in the modulation of several genes in the glial cells, including anti-inflammatory genes, and neuronal activation markers. A mild increase in cleav Casp3 expression in TG and c-Fos and GFAP in the spinal trigeminal nucleus of CGRP injected animals was observed. These data provide evidence that glial cells can participate in neuroimmune interaction due to CGRP in the TG via NF-κB signaling pathway. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
satellite glial cells |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
calcitonin gene related peptide (CGRP) |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
nuclear factor kappa B (NF-κB) |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
trigeminal ganglion |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
glial cells |
| 書誌情報 |
en : International Journal of Molecular Sciences
巻 21,
号 17,
p. 6005,
発行日 2020-08-20
|
| 収録物ID |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
14220067 |
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
16616596 |
| 出版者 |
|
|
出版者 |
MDPI |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
| EID |
|
|
識別子 |
368866 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
ijms_21_17_6005.pdf (2.85 MB)
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