| Item type |
文献 / Documents(1) |
| 公開日 |
2020-09-04 |
| アクセス権 |
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|
アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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|
関連識別子 |
https://doi.org/10.1016/j.job.2020.01.002 |
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関連名称 |
10.1016/j.job.2020.01.002 |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| タイトル |
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|
タイトル |
PLAG1 enhances the stemness profiles of acinar cells in normal human salivary glands in a cell type-specific manner |
| 著者 |
ゴトウ, ユリコ
イビ, ミホ
サトウ, ヒロタカ
タナカ, ジュンイチ
ヤスハラ, リカ
青田, 桂子
東, 雅之
フカダ, トシユキ
ミシマ, ケンジ
イリエ, タロウ
|
| 抄録 |
|
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内容記述 |
Objectives: Details of the histogenesis of salivary gland tumors are largely unknown. The oncogenic role of PLAG1 in the salivary gland has been demonstrated in vivo. Herein, we demonstrate the roles of PLAG1 in the acinar and ductal cells of normal human salivary glands in an attempt to clarify the early events that occur during the histogenesis of salivary gland tumors.
Methods: Normal salivary gland cells with acinar- (NS-SV-AC) and ductal- (NS-SV-DC) phenotypes were transfected with PLAG1 plasmid DNA. Subsequently, the PLAG1 overexpressed and mock cells were examined by cell proliferation, transwell migration, and salisphere formation assays. The expression levels of salivary and pluripotent stem cell markers and differentiation markers were evaluated by quantitative real-time polymerase chain reaction and immunofluorescence. Alterations in transcriptional expressions were investigated via cap analysis of gene expression with gene-enrichment and functional annotation analysis.
Results: PLAG1 promoted cell proliferation and transwell migration in the acinar and ductal cells, and markedly enhanced the stemness profiles and luminal cell-like profiles in acinar cells; the stemness profiles were partially increased in the ductal cells.
Conclusion: PLAG1 enhanced the stemness profiles in the acinar cells of normal human salivary glands in a cell type-specific manner. Thus, it may be involved in salivary gland tumorigenesis by increasing the stemness character of the normal salivary gland cells. |
| キーワード |
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|
主題 |
Salivary gland neoplasm |
| キーワード |
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|
主題 |
Tumorigenesis |
| キーワード |
|
|
主題 |
Pleomorphic adenoma gene 1 |
| キーワード |
|
|
主題 |
Stemness |
| キーワード |
|
|
主題 |
Acinar cell |
| 書誌情報 |
en : Journal of Oral Biosciences
巻 62,
号 1,
p. 99-106,
発行日 2020-01-30
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13490079 |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11896386 |
| 出版者 |
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|
出版者 |
The Japanese Association for Oral Biology |
| 出版者 |
|
|
出版者 |
Elsevier |
| 権利情報 |
|
|
権利情報 |
© 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| EID |
|
|
識別子 |
363778 |
| 言語 |
|
|
言語 |
eng |
job_62_1_99.pdf (999 KB)
