| Item type |
文献 / Documents(1) |
| 公開日 |
2021-01-15 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
関連識別子 |
https://doi.org/10.1371/journal.pone.0233390 |
|
|
関連名称 |
10.1371/journal.pone.0233390 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Interferon regulatory factor 7 mediates obesity-associated MCP-1 transcription |
| タイトル別表記 |
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その他のタイトル |
IRF7 mediates MCP-1 in adipocyte |
| 著者 |
黒田, 雅士
ニシグチ, ミサ
ウガワ, ナホ
イシカワ, エツコ
カワバタ, ヤスヨ
オカモト, サヤ
ササキ, ワカ
ミヤタケ, ユミコ
セベ, マユ
升本, 早枝子
堤, 理恵
原田, 永勝
阪上, 浩
|
| 抄録 |
|
|
内容記述 |
Hypertrophy, associated with adipocyte dysfunction, causes increased pro-inflammatory adipokine, and abnormal glucose and lipid metabolism, leading to insulin resistance and obesity-related-health problems. By combining DNA microarray and genomic data analyses to predict DNA binding motifs, we identified the transcription factor Interferon Regulatory Factor 7 (IRF7) as a possible regulator of genes related to adipocyte hypertrophy. To investigate the role of IRF7 in adipocytes, we examined gene expression patterns in 3T3-L1 cells infected with a retrovirus carrying the IRF7 gene and found that enforced IRF7 expression induced the expression of monocyte chemoattractant protein-1 (MCP-1), a key initial adipokine in the chronic inflammation of obesity. CRISPR/Cas9 mediated-suppression of IRF7 significantly reduced MCP-1 mRNA. Luciferase assays, chromatin immunoprecipitation PCR analysis and gel shift assay showed that IRF7 transactivates the MCP-1 gene by binding to its proximal Interferon Stimulation Response Element (ISRE), a putative IRF7 binding motif. IRF7 knockout mice exhibited lower expression of MCP-1 in epidydimal white adipose tissue under high-fat feeding conditions, suggesting the transcription factor is physiologically important for inducing MCP-1. Taken together, our results suggest that IRF7 transactivates MCP-1 mRNA in adipocytes, and it may be involved in the adipose tissue inflammation associated with obesity. |
| 書誌情報 |
en : PLOS ONE
巻 15,
号 5,
p. e0233390,
発行日 2020-05-21
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
19326203 |
| 出版者 |
|
|
出版者 |
PLOS |
| 権利情報 |
|
|
権利情報 |
© 2020 Kuroda et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| EID |
|
|
識別子 |
367803 |
| 言語 |
|
|
言語 |
eng |
pone_15_5_e0233390.pdf (2.78 MB)
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