| Item type |
文献 / Documents(1) |
| 公開日 |
2021-02-16 |
| アクセス権 |
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|
アクセス権 |
open access |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
https://doi.org/10.1242/jcs.195263 |
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|
言語 |
ja |
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|
関連名称 |
10.1242/jcs.195263 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Mouse Stbd1 is N-myristoylated and affects ER–mitochondria association and mitochondrial morphology |
|
言語 |
en |
| 著者 |
Demetriadou, Anthi
Morales-Sanfrutos, Julia
Nearchou, Marianna
馬場, 麻人
Kyriacou, Kyriacos
Tate, Edward W.
Drousiotou, Anthi
Petrou, Petros P.
|
| 抄録 |
|
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内容記述タイプ |
Abstract |
|
内容記述 |
Starch binding domain-containing protein 1 (Stbd1) is a carbohydrate-binding protein that has been proposed to be a selective autophagy receptor for glycogen. Here, we show that mouse Stbd1 is a transmembrane endoplasmic reticulum (ER)-resident protein with the capacity to induce the formation of organized ER structures in HeLa cells. In addition to bulk ER, Stbd1 was found to localize to mitochondria-associated membranes (MAMs), which represent regions of close apposition between the ER and mitochondria. We demonstrate that N-myristoylation and binding of Stbd1 to glycogen act as major determinants of its subcellular targeting. Moreover, overexpression of non-myristoylated Stbd1 enhanced the association between ER and mitochondria, and further induced prominent mitochondrial fragmentation and clustering. Conversely, shRNA-mediated Stbd1 silencing resulted in an increase in the spacing between ER and mitochondria, and an altered morphology of the mitochondrial network, suggesting elevated fusion and interconnectivity of mitochondria. Our data unravel the molecular mechanism underlying Stbd1 subcellular targeting, support and expand its proposed function as a selective autophagy receptor for glycogen and uncover a new role for the protein in the physical association between ER and mitochondria. |
|
言語 |
en |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Stbd1 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Glycogen |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Endoplasmic reticulum |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Mitochondria |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
N-myristoylation |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Mitochondria-associated membranes |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Organized smooth endoplasmic reticulum |
| 書誌情報 |
en : Journal of Cell Science
巻 130,
号 5,
p. 903-915,
発行日 2017-03-01
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
00219533 |
| 収録物ID |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
14779137 |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00694823 |
| 収録物ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11720335 |
| 出版者 |
|
|
出版者 |
The Company of Biologists |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
| EID |
|
|
識別子 |
327256 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
jcs_130_5_903.pdf (13.4 MB)
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