| Item type |
文献 / Documents(1) |
| 公開日 |
2021-02-19 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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|
関連識別子 |
https://doi.org/10.1262/jrd.2019-049 |
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関連名称 |
10.1262/jrd.2019-049 |
| 出版タイプ |
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|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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|
タイトル |
The effects of electroporation on viability and quality of in vivo-derived bovine blastocysts |
| タイトル別表記 |
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その他のタイトル |
ELECTROPORATION OF BOVINE BLASTOCYSTS |
| 著者 |
谷原, 史倫
平田, 真樹
モリカワ, シゲキ
Nguyen, Nhien Thi
Le, Quynh Anh
ヒラノ, タカユキ
フクミ, ヨシユキ
アベ, トシアキ
音井, 威重
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| 抄録 |
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内容記述 |
The introduction of exogenous molecules into embryos is required for analyses of molecular dynamics and specific gene functions during early embryonic development. Electroporation is an effective method to transport exogenous molecules into cells, but is rarely used in bovine embryos. First, we evaluated the viability of in vivo-derived bovine blastocysts after electroporation with fluorescein (FAM) labeled-oligonucleotides with varying pulse numbers (3, 5, 7, and 10), while keeping the pulse duration at 1 msec and the electric field of 20 V/mm. Next, we examined the effects of zona pellucida status on blastocyst quality after electroporation, by comparing the average diameter of blastocysts before and after electroporation using blastocysts with intact zona pellucida and hatching/hatched blastocysts. Electroporation successfully introduced exogenous molecules into in vivo-derived bovine blastocysts without loss of viability. Moreover, the status of the zona pellucida may be associated with the quality of blastocysts after electroporation. |
| キーワード |
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|
主題 |
Bovine |
| キーワード |
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|
主題 |
Blastocyst |
| キーワード |
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|
主題 |
Electroporation |
| キーワード |
|
|
主題 |
In vivo |
| キーワード |
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|
主題 |
Zona pellucida |
| 書誌情報 |
en : Journal of Reproduction and Development
巻 65,
号 5,
p. 475-479,
発行日 2019
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
13484400 |
| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
09168818 |
| 収録物ID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10936678 |
| 出版者 |
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出版者 |
Society for Reproduction and Development |
| 権利情報 |
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|
権利情報 |
This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) |
| EID |
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|
識別子 |
356993 |
| 言語 |
|
|
言語 |
eng |
jrd_65_5_475.pdf (980 KB)
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