| Item type |
文献 / Documents(1) |
| 公開日 |
2021-03-04 |
| アクセス権 |
|
|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1161/JAHA.118.011824 |
|
|
言語 |
ja |
|
|
関連名称 |
10.1161/JAHA.118.011824 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
Role of Perivascular Oligodendrocyte Precursor Cells in Angiogenesis After Brain Ischemia |
|
言語 |
en |
| タイトル別表記 |
|
|
その他のタイトル |
Role of Hypoxic OPC in Angiogenesis |
|
言語 |
en |
| 著者 |
キシダ, ナツエ
マキ, タカクニ
高木, 康志
ヤスダ, ケン
キノシタ, ヒサノリ
アヤキ, タカシ
ノロ, タカユキ
キノシタ, ユウスケ
オノ, ユウイチ
カタオカ, ヒロハル
ヨシダ, カズミチ
Lo, Eng H.
アライ, ケン
ミヤモト, ススム
タカハシ, リョウスケ
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Background－Oligodendrocyte precursor cells (OPCs) regulate neuronal, glial, and vascular systems in diverse ways and display phenotypic heterogeneity beyond their established role as a reservoir for mature oligodendrocytes. However, the detailed phenotypic changes of OPCs after cerebral ischemia remain largely unknown. Here, we aimed to investigate the roles of reactive OPCs in the ischemic brain.
Methods and Results－The behavior of OPCs was evaluated in a mouse model of ischemic stroke produced by transient middle cerebral artery occlusion in vivo. For in vitro experiments, the phenotypic change of OPCs after oxygen glucose derivation was examined using a primary rat OPC culture. Furthermore, the therapeutic potential of hypoxic OPCs was evaluated in a mouse model of middle cerebral artery occlusion in vivo. Perivascular OPCs in the cerebral cortex were increased alongside poststroke angiogenesis in a mouse model of middle cerebral artery occlusion. In vitro RNA‐seq analysis revealed that primary cultured OPCs increased the gene expression of numerous pro‐angiogenic factors after oxygen glucose derivation. Hypoxic OPCs secreted a greater amount of pro‐angiogenic factors, such as vascular endothelial growth factor and angiopoietin‐1, compared with normoxic OPCs. Hypoxic OPC‐derived conditioned media increased the viability and tube formation of endothelial cells. In vivo studies also demonstrated that 5 consecutive daily treatments with hypoxic OPC‐conditioned media, beginning 2 days after middle cerebral artery occlusion, facilitated poststroke angiogenesis, alleviated infarct volume, and improved functional disabilities.
Conclusions－Following cerebral ischemia, the phenotype of OPCs in the cerebral cortex shifts from the parenchymal subtype to the perivascular subtype, which can promote angiogenesis. The optimal use of hypoxic OPCs secretome would provide a novel therapeutic option for stroke. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
angiogenesis |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
glial cell |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
stem cell |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
stroke |
| 書誌情報 |
en : Journal of the American Heart Association
巻 8,
号 9,
p. e011824,
発行日 2019-04-25
|
| 収録物ID |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
20479980 |
| 出版者 |
|
|
出版者 |
The American Heart Association |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
© 2019 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
| EID |
|
|
識別子 |
366187 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
jaha_8_9_e011824.pdf (4.18 MB)
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