| Item type |
文献 / Documents(1) |
| 公開日 |
2021-02-22 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.1292/jvms.15-0658 |
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関連名称 |
10.1292/jvms.15-0658 |
| 出版タイプ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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|
タイトル |
The optimal period of Ca-EDTA treatment for parthenogenetic activation of porcine oocytes during maturation culture |
| タイトル別表記 |
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その他のタイトル |
ACTIVATION OF PORCINE OOCYTES BY CALCIUM-EDTA |
| 著者 |
モリタ, ヤスヒロ
タニグチ, マサヤス
谷原, 史倫
イトウ, アヤ
Namula, Zhao
Do, Lanh Thi Kim
タカギ, ミツヒロ
竹本, 龍也
音井, 威重
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| 抄録 |
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内容記述 |
The changes triggered by sperm-induced activation of oocytes, which are required for normal oocyte development, can be mediated by other agents, thereby inducing the parthenogenesis. In this study, we exposed porcine oocytes to 1 mM Ca-EDTA, a metal-ion chelator, at various intervals during 48 hr of in vitro maturation to determine the optimum period of Ca-EDTA treatment for parthenogenetic activation. When the oocytes were cultured with or without Ca-EDTA from 36 hr (post-12), 24 hr (post-24), 12 hr (post-36) and 0 hr (post-48) after the start of maturation culture, the blastocyst formation rates were significantly higher (P<0.05) in the post-24, post-36 and post-48 groups (3.3%, 4.0% and 2.6%, respectively) than those in the control group without treatment (0%). Furthermore, when the oocytes were cultured with Ca-EDTA for 0 hr (control), 12 hr (pre-12), 24 hr (pre-24), 36 hr (pre-36) and 48 hr (pre-48) from the start of maturation culture, the oocytes formed blastocysts only in the pre-36 and pre-48 groups (0.4% or 0.8%, respectively). Pronuclei (<66.7%) were observed only when the periods of Ca-EDTA treatment were more than 12 hr during maturation culture. In the control group, no pronuclei were detected. Our findings demonstrate that porcine immature oocytes can be parthenogenetically activated by Ca-EDTA treatment for at least 24 hr to 36 hr during maturation culture, leading to pronucleus formation followed by the formation of blastocysts. |
| キーワード |
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主題 |
blastocyst |
| キーワード |
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|
主題 |
Ca-EDTA |
| キーワード |
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|
主題 |
nuclear status |
| キーワード |
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|
主題 |
parthenogenesis |
| キーワード |
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|
主題 |
porcine oocyte |
| 書誌情報 |
en : Journal of Veterinary Medical Science
巻 78,
号 6,
p. 1019-1023,
発行日 2016-03-04
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
13477439 |
| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
09167250 |
| 収録物ID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10796138 |
| 出版者 |
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出版者 |
The Japanese Society of Veterinary Science |
| 権利情報 |
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|
権利情報 |
This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License <http://creativecommons.org/licenses/by-nc-nd/4.0/>. |
| EID |
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|
識別子 |
312016 |
| 言語 |
|
|
言語 |
eng |
jvms_78_6_1019.pdf (504 KB)
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