| Item type |
文献 / Documents(1) |
| 公開日 |
2021-04-19 |
| アクセス権 |
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|
アクセス権 |
open access |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1111/nan.12288 |
|
|
言語 |
ja |
|
|
関連名称 |
10.1111/nan.12288 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
|
|
タイトル |
The identification of raft‐derived tau‐associated vesicles that are incorporated into immature tangles and paired helical filaments |
|
言語 |
en |
| タイトル別表記 |
|
|
その他のタイトル |
Raft-derived tau-associated vesicles |
|
言語 |
en |
| 著者 |
ニシカワ
タカハシ
ナカモリ
ホソミ
マルヤマ
宮﨑, 由道
和泉, 唯信
マツモト
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Aims: Neurofibrillary tangles (NFTs), a cardinal pathological feature of neurodegenerative disorders, such as Alzheimer's disease (AD) are primarily composed of hyper‐phosphorylated tau protein. Recently, several other molecules, including flotillin‐1, phosphatidylinositol‐4,5‐bisphosphate [PtdIns(4,5)P2] and cyclin‐dependent kinase 5 (CDK5), have also been revealed as constituents of NFTs. Flotillin‐1 and PtdIns(4,5)P2 are considered markers of raft microdomains, whereas CDK5 is a tau kinase. Therefore, we hypothesized that NFTs have a relationship with raft domains and the tau phosphorylation that occurs within NFTs. Methods: We investigated six cases of AD, six cases of other neurodegenerative diseases with NFTs and three control cases. We analysed the PtdIns(4,5)P2‐immunopositive material in detail, using super‐resolution microscopy and electron microscopy to elucidate its pattern of expression. We also investigated the spatial relationship between the PtdIns(4,5)P2‐immunopositive material and tau kinases through double immunofluorescence analysis. Results: Pretangles contained either paired helical filaments (PHFs) or PtdIns(4,5)P2‐immunopositive small vesicles (approximately 1 μm in diameter) with nearly identical topology to granulovacuolar degeneration (GVD) bodies. Various combinations of these vesicles and GVD bodies, the latter of which are pathological hallmarks observed within the neurons of AD patients, were found concurrently in neurons. These vesicles and GVD bodies were both immunopositive not only for PtdIns(4,5)P2, but also for several tau kinases such as glycogen synthase kinase‐3β and spleen tyrosine kinase. Conclusions: These observations suggest that clusters of raft‐derived vesicles that resemble GVD bodies are substructures of pretangles other than PHFs. These tau kinase‐bearing vesicles are likely involved in the modification of tau protein and in NFT formation. |
|
言語 |
en |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Alzheimer’s disease |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
granulovacuolar degeneration |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
lipid raft |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
pretangle |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
signaling endosome |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
tau |
| 書誌情報 |
en : Neuropathology and Applied Neurobiology
巻 42,
号 7,
p. 639-653,
発行日 2015-10-26
|
| 収録物ID |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13652990 |
| 収録物ID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00754798 |
| 出版者 |
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|
出版者 |
British Neuropathological Society |
|
言語 |
en |
| 出版者 |
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|
出版者 |
John Wiley & Sons |
|
言語 |
en |
| 権利情報 |
|
|
言語 |
en |
|
権利情報 |
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
| EID |
|
|
識別子 |
307161 |
|
識別子タイプ |
URI |
| 言語 |
|
|
言語 |
eng |
nan_42_7_639.pdf (1.33 MB)
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