| Item type |
文献 / Documents(1) |
| 公開日 |
2021-07-09 |
| アクセス権 |
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アクセス権 |
open access |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 出版社版DOI |
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関連識別子 |
https://doi.org/10.3892/ol.2018.8887 |
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関連名称 |
10.3892/ol.2018.8887 |
| 出版タイプ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| タイトル |
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|
タイトル |
Effects of TONS504‑photodynamic therapy on mouse mammary tumor cells |
| タイトル別表記 |
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その他のタイトル |
TONS504-PHOTODYNAMIC THERAPY INDUCES CYTOTOXIC EFFECTS IN EMT6 CELLS |
| 著者 |
オオサキ, トモヒロ
サカタ, イサオ
宇都, 義浩
ヤマシタ, マサミチ
ムラハタ, ユウスケ
アズマ, カズオ
ツカ, タケシ
イトウ, ノリヒコ
イマガワ, トモヒロ
オカモト, ヨシハル
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| 抄録 |
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内容記述 |
In the present study, TONS504 (C51H58N8O5I2; molecular weight, 1,116.9), a novel cationic hydrophilic photosensitizer, was synthesized from protoporphyrin IX dimethyl ester through a five‑step process according to a patented method for use in photodynamic therapy (PDT). The subcellular localization of TONS504 and the cytotoxic effects of TONS504‑mediated PDT in the mouse mammary tumor EMT6 cell line were investigated. TONS504 was localized primarily in the lysosomes and partially in the mitochondria. The cytotoxic effects of TONS504‑mediated PDT in the mouse mammary tumor EMT6 cell line were investigated using a WST8 assay and an Oxidative Stress kit. The cell viability values following treatment with 10 µg/ml TONS504 at light energies of 0, 1, 5 and 10 J/cm2 were 92.5, 101.8, 27.7 and 1.8%, respectively. The percentages of reactive oxygen species (ROS)(+) cells following the same treatment were 8.6, 8.5, 29.2 and 70.1%, respectively, whereas the percentages of apoptotic cells were 7.1, 5.6, 24.8 and 48.7%, respectively. The percentages of ROS(+) and apoptotic cells in the group subjected to TONS504‑mediated PDT increased in a manner dependent on the TONS504 concentration and light energy. Further studies are required to evaluate the in vivo pharmacokinetics, tissue distribution and photodynamic effects of TONS504. |
| キーワード |
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主題 |
apoptosis |
| キーワード |
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|
主題 |
mammary tumor |
| キーワード |
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|
主題 |
photodynamic therapy |
| キーワード |
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|
主題 |
TONS504 |
| キーワード |
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|
主題 |
reactive oxygen species |
| 書誌情報 |
en : Oncology Letters
巻 16,
号 2,
p. 2078-2084,
発行日 2018-06-04
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| 収録物ID |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
17921074 |
| 収録物ID |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
17921082 |
| 出版者 |
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出版者 |
Spandidos Publications |
| 権利情報 |
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|
権利情報 |
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) License. |
| EID |
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|
識別子 |
355615 |
| 言語 |
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|
言語 |
eng |
ol_16_2_2078.pdf (1.23 MB)
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