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Involvement of butyrate in electrogenic K+ secretion in rat rectal colon
https://tokushima-u.repo.nii.ac.jp/records/2008607
https://tokushima-u.repo.nii.ac.jp/records/2008607bab5299b-3c0e-4f17-8df8-6b15afa04e4d
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
paejp_471_2_313.pdf (4.93 MB)
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| Item type | 文献 / Documents(1) | |||||||||||||||||||||||||
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| 公開日 | 2021-04-22 | |||||||||||||||||||||||||
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| アクセス権 | open access | |||||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||||||
| 出版社版DOI | ||||||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1007/s00424-018-2208-y | |||||||||||||||||||||||||
| 関連名称 | 10.1007/s00424-018-2208-y | |||||||||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||||||
| タイトル | ||||||||||||||||||||||||||
| タイトル | Involvement of butyrate in electrogenic K+ secretion in rat rectal colon | |||||||||||||||||||||||||
| 著者 |
稲垣, 明浩
× 稲垣, 明浩
WEKO
1249
× ハヤシ, ミキオ
× Andharia, Naaz
× マツダ, ヒロコ
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| 内容記述 | Short-chain fatty acids (SCFAs), such as acetate, propionate, and butyrate, are synthesized from dietary carbohydrates by colonic bacterial fermentation. These SCFAs supply energy, suppress cancer, and affect ion transport. However, their roles in ion transport and regulation in the intracellular environment remain unknown. In order to elucidate the roles of SCFAs, we measured short-circuit currents (ISC) and performed RT-PCR and immunohistochemical analyses of ion transporters in rat rectal colon. The application of 30 mM butyrate shifted ISC in a negative direction, but did not attenuate the activity of epithelial Na+ channels (ENaC). The application of bumetanide, a Na+-K+-2Cl− cotransporter inhibitor, to the basolateral side reduced the negative ISC shift induced by butyrate. The application of XE991, a KCNQ-type K+ channel inhibitor, to the apical side decreased the ISC shift induced by butyrate in a dose-dependent manner. The ISC shift was independent of HCO3− and insensitive to ibuprofen, an SMCT1 inhibitor. The mucosa from rat rectal colon expressed mRNAs of H+-coupled monocarboxylate transporters (MCT1, MCT4, and MCT5, also referred to as SLC16A1, SLC16A3, and SLC16A4, respectively). RT-PCR and immunofluorescence analyses demonstrated that KCNQ2 and KCNQ4 localized to the apical membrane of surface cells in rat rectal colon. These results indicate that butyrate, which may be transported by H+-coupled monocarboxylate transporters, activates K+ secretion through KCNQ-type K+ channels on the apical membrane in rat rectal colon. KCNQ-type K+ channels may play a role in intestinal secretion and defense mechanisms in the gastrointestinal tract. | |||||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||||
| 主題 | Short-chain fatty acid | |||||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||||
| 主題 | Rectal colon | |||||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||||
| 主題 | Short-circuit current | |||||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||||
| 主題 | KCNQ | |||||||||||||||||||||||||
| 書誌情報 |
en : Pflügers Archiv: European Journal of Physiology 巻 471, 号 2, p. 313-327, 発行日 2018-09-25 |
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| 収録物識別子タイプ | ISSN | |||||||||||||||||||||||||
| 収録物識別子 | 00316768 | |||||||||||||||||||||||||
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| 収録物識別子タイプ | ISSN | |||||||||||||||||||||||||
| 収録物識別子 | 14322013 | |||||||||||||||||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||||||||||||||||
| 収録物識別子 | AA00771833 | |||||||||||||||||||||||||
| 出版者 | ||||||||||||||||||||||||||
| 出版者 | Springer Nature | |||||||||||||||||||||||||
| 権利情報 | ||||||||||||||||||||||||||
| 権利情報 | This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. | |||||||||||||||||||||||||
| EID | ||||||||||||||||||||||||||
| 識別子 | 355069 | |||||||||||||||||||||||||
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| 言語 | eng | |||||||||||||||||||||||||
